G-CSF Rat Protein
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Specifications
Product Data | |
Species | Rat |
Expression Host | E. coli |
Expression cDNA Clone or AA Sequence |
MKKIPLLTVS SLPPSLPLPR SFLLKSLEQV RKIQARNTEL LEQLCATYKL CHPEELVLFG HSLGIPKASL SSCSSQALQQ TKCLSQLHSG LFLYQGLLQA LAGISSELAP TLDMLHLDVD NFATTIWQQM ESLGVAPTVQ PTQSTMPIFT SAFQRRAGGV LVTSYLQSFL ETAHHALHHL PRPAQKHFPE SLFISI
|
Predicted MW | 21.6 kDa |
Purity | >98% pure by SDS-PAGE gel and HPLC analyses |
Buffer | Presentation State: Purified State: Lyophilized (0.2µ Sterile filtered) purified protein Buffer System: 10mM Sodium Citrate, pH 4.0 |
Bioactivity | Biological: Determined by its ability to stimulate the proliferation of Mouse NFS-60 cells. The expected ED50 is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg. Specific: ≥ 2 x 107 units/mg. |
Endotoxin | < 0.1 ng/μg of protein (< 1EU/μg) |
Reconstitution | Restore in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
Preparation | Lyophilized (0.2µ Sterile filtered) purified protein |
Protein Description | Recombinant Rat G-CSF is a 21.6 kDa protein consisting of 196 amino acid residues. |
Note | Centrifuge the vial prior to opening. |
Storage | Prior to reconstitution store at 2-8°C for 6 months, at RT for one month or at -20°C to -80°C for longer. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Reference Data | |
RefSeq | NP_058800 |
Locus ID | 25610 |
Cytogenetics | 10q31 |
Synonyms | Gcsf |
Summary | putative hematopoetic growth factor for neutrophils; used as a treatment for cyclic hematopoesis in humans and dogs [RGD, Feb 2006] |
Documents
FAQs |
Resources
Recombinant Protein Resources |
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