Dickkopf-3 (DKK3) Human Protein

CAT#: AR31165PU-N

Dickkopf-3 (DKK3) human recombinant protein, 10 µg

Specifications

Product Data

• Species: Human
• Expression Host: CHO
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  APAPTATSAP VKPGPALSYP QEEATLNEMF REVEELMEDT QHKLRSAVEE MEAEEAAAKA SSEVNLANLP PSYHNETNTD TKVGNNTIHV HREIHKITNN QTGQMVFSET VITSVGDEEG RRSHECIIDE DCGPSMYCQF ASFQYTCQPC RGQRMLCTRD SECCGDQLCV WGHCTKMATR GSNGTICDNQ RDCQPGLCCA FQRGLLFPVC TPLPVEGELC HDPASRLLDL ITWELEPDGA LDRCPCASGL LCQPHSHSLV YVCKPTFVGS RDQDGEILLP REVPDEYEVG SFMEEVRQEL EDLERSLTEE MALREPAAA AAALLGGEEI
• Predicted MW: 39-49 kDa
• Purity: >98% by SDS-PAGE & HPLC analysis
• Buffer: Presentation State: Purified
  State: Lyophilized purified protein
  Stabilizer: None
• Bioactivity: Biological: Determined by its ability to inhibit alkaline phosphatase activity in differentiating MC3T3 E1 cells. The expected ED50 for this effect is 2.0–4.0 ng/ml.
• Reconstitution: We recommended a quick spin followed by reconstitution in water to a concentration of 0.1-1.0 mg/ml.
  This solution can be diluted into other aqueous buffers and stored at 4°C for one week or at –20°C for future use.
• Preparation: Lyophilized purified protein
• Protein Description: Recombinant human DKK-3 expressed in CHO cells is a glycoprotein that has a calculated molecular weight of 36.3 kDa and contains 329 amino acid residues. Due to glycosylation, human DKK-3 migrates at an apparent molecular weight of approximately 39-49 kDa by SDS-PAGE analysis under non-reducing conditions.
• Note: Centrifuge vials before opening!
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_001347186
• Locus ID: 50781
• Cytogenetics: 7 F1
• Synonyms: AW061014; C87148; dkk-3; mDkk-3