Interleukin-27 / IL27A Human Protein
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Specifications
Product Data | |
Species | Human |
Expression Host | HEK293 |
Expression cDNA Clone or AA Sequence |
p28 subunit: FPRPPGRPQL SLQELRREFT VSLHLARKLL SEVRGQAHRF AESHLPGVNL YLLPLGEQLP DVSLTFQAWR RLSDPERLCF ISTTLQPFHA LLGGLGTQGR WTNMERMQLW AMRLDLRDLQ RHLRFQVLAA GFNLPEEEEE EEEEEEEERK GLLPGALGSA LQGPAQVSWP QLLSTYRLLH SLELVLSRAV RELLLLSKAG HSVWPLGFPT LSPQP
EBI3 subunit: RKGPPAALTL PRVQCRASRY PIAVDCSWTL PPAPNSTSPV SFIATYRLGM AARGHSWPCL QQTPTSTSCT ITDVQLFSMA PYVLNVTAVH PWGSSSSFVP FITEHIIKPD PPEGVRLSPL AERQLQVQWE PPGSWPFPEI FSLKYWIRYK RQGAARFHRV GPIEATSFIL RAVRPRARYY VQVAAQDLTD YGELSDWSLP ATATMSLGK |
Predicted MW | 47.8 kDa |
Purity | >90% pure by SDS-PAGE gel and HPLC analyses |
Buffer | Presentation State: Purified State: Lyophilized (0.2μ Sterile filtered) purified protein Buffer System: 10mM Sodium Phosphate, pH 6.5 + 100mM NaCl |
Bioactivity | Biological: Determined by its ability to stimulate the proliferation of Human TF-1 cells. The expected ED50 range is 400-500 ng/ml. |
Endotoxin | < 0.1 ng/μg of protein (<1EU/μg) |
Reconstitution | Restore in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
Preparation | Lyophilized (0.2μ Sterile filtered) purified protein |
Protein Description | Recombinant Human IL-27 produced from HEK293 cells is a non-disulfide-linked, heterodimeric protein composed of a 209 amino acid length EBI3 subunit and a 215 amino acid length IL-27-p28 subunit, for a total sequence length of 424 amino acid residues. The calculated molecular weight of the associated IL-27 subunits is 47.8 kDa. |
Storage | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Reference Data | |
RefSeq | NP_663634 |
Locus ID | 246778 |
UniProt ID | Q8NEV9 |
Cytogenetics | 16p12.1-p11.2 |
Synonyms | IL-27; IL-27A; IL27A; IL27p28; IL30; p28 |
Summary | The protein encoded by this gene is one of the subunits of a heterodimeric cytokine complex. This protein is related to interleukin 12A (IL12A). It interacts with Epstein-Barr virus induced gene 3 (EBI3), a protein similar to interleukin 12B (IL12B), and forms a complex that has been shown to drive rapid expansion of naive but not memory CD4(+) T cells. The complex is also found to synergize strongly with interleukin 12 to trigger interferon gamma (IFNG) production of naive CD4(+) T cells. The biological effects of this cytokine are mediated by the class I cytokine receptor (WSX1/TCRR). [provided by RefSeq, Jul 2008] |
Protein Families | Druggable Genome, Transmembrane |
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