BMP2 / BMP2A (CHO cell derived) Human Protein

CAT#: AR31188PU-S

BMP2 / BMP2A (CHO cell derived) human recombinant protein, 2 µg

Specifications

Product Data

• Species: Human
• Expression Host: CHO
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  QAKHKQRKRL KSSCKRHPLY VDFSDVGWND WIVAPPGYHA FYCHGECPFP LADHLNSTNH AIVQTLVNSV NSKIPKACCV PTELSAISML YLDENEKVVL KNYQDMVVEG CGCR
• Predicted MW: 26.0 kDa
• Purity: >95% pure by SDS-PAGE gel and HPLC analyses.
• Buffer: Presentation State: Purified
  State: Lyophilized (sterile filtered) purified protein
• Bioactivity: Biological: Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED₅₀ for this effect is 40-100 ng/ml.
  
• Endotoxin: < 0.1 ng/µg (1EU/µg)
• Reconstitution: Restore in water to a concentration of 0.1-1.0 mg/ml.
  This solution can then be diluted into other aqueous buffers and stored at 2-8°C for 1 week or -20°C for future use.
• Preparation: Lyophilized (sterile filtered) purified protein
• Protein Description: Recombinant Human BMP-2 derived from CHO cells is a homodimeric glycoprotein that consists of two 114 amino acid polypeptide chains linked by a single disulfide bond. Due to glycosylation, CHO cell-derived Human BMP-2 migrates at an apparent molecular weight of approximately 28-29 kDa by SDS-PAGE analysis under non-reducing conditions.
  Mature sequence is complete identical with Mouse and Rat.
• Note: Centrifuge the vial prior to opening!
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_001191
• Locus ID: 650
• UniProt ID: P12643, C8C060
• Cytogenetics: 20p12.3
• Synonyms: BDA2; BMP2A; SSFSC
• Summary: 'This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer, which plays a role in bone and cartilage development. Duplication of a regulatory region downstream of this gene causes a form of brachydactyly characterized by a malformed index finger and second toe in human patients. [provided by RefSeq, Jul 2016]'
• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Secreted Protein, Stem cell relevant signaling - TGFb/BMP signaling pathway, Transmembrane
• Protein Pathways: Acute myeloid leukemia, Basal cell carcinoma, Cytokine-cytokine receptor interaction, Endocytosis, Hedgehog signaling pathway, Hematopoietic cell lineage, Melanogenesis, Pathways in cancer, TGF-beta signaling pathway