Interleukin-12 (ILA 23-219+ILB 23-328) Human Protein

CAT#: AR51663PU-N

Interleukin-12 (ILA 23-219+ILB 23-328) human recombinant protein, 0.25 mg

Product Images

Specifications

Product Data

• Species: Human
• Expression Host: Insect
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  * IL-12 is a heterodimer of IL12A and IL12B linked through a disulfide-bond between cysteines in Bold in below sequences.
  > IL12A(p35)
  RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTK NESCLNSRET SFITNGSCLA SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNMLAV IDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNASHHH HHH
  
  > IL12B(p40)
  IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS
• Concentration: lot specific
• Purity: >90% by SDS - PAGE
• Buffer: Presentation State: Purified
  State: Liquid purified protein
  Buffer System: Phosphate buffered saline (pH7.4), 10% glycerol
• Bioactivity: Biological: Human IL12 stimulates IFN-g secretion of the NK-92 human natural killer cells. The ED50 range ≤ 1 ng/ml.
• Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation: Liquid purified protein
• Protein Description: Recombinant Human IL12 protein, fused to His-tag at C-terminus, was expressed in insect cells using baculovirus expression system and purified by using conventional chromatography techniques.
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_000873
• Locus ID: 3592
• UniProt ID: P29459, O60595
• Cytogenetics: 3q25.33
• Synonyms: CLMF; IL-12A; NFSK; NKSF1; P35
• Summary: 'This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. [provided by RefSeq, Jul 2008]'
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Allograft rejection, Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway, Type I diabetes mellitus