CD309 / VEGFR-2 / Flk-1 (D1-D7) Human Protein

CAT#: DA3530X

CD309 / VEGFR-2 / Flk-1 (D1-D7) human recombinant protein, 50 µg

Product Images

Specifications

Product Data

• Species: Human
• Expression Host: Insect
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  ASVGLPSVSLDLPRLSIQKDILTIKANTTLQITCRGQRDLDWLWPNNQSGSEQRVEVTECSDGLFCKTLTIPKVIGNDTGAYKCFYRETDLASVIYVYVQDYRSPFIASVSDQHGVVYITENKNKTVVIPCLGSISNLNVSLCARYPEKRFVPDGNRISWDSKKGFTIPSYMISYAGMVFCEAKINDESYQSIMYIVVVVGYRIYDVVLSPSHGIELSVGEKLVLNCTARTELNVGIDFNWEYPSSKHQHKKLVNRDLKTQSGSEMKKFLSTLTIDGVTRSDQGLYTCAASSGLMTKKNSTFVRVHEKPFVAFGSGMESLVEATVGERVRIPAKYLGYPPPEIKWYKNGIPLESNHTIKAGHVLTIMEVSERDTGNYTVILTNPISKEKQSHVVSLVVYVPPQIGEKSLISPVDSYQYGTTQTLTCTVYAIPPPHHIHWYWQLEEECANEPSQAVSVTNPYPCEEWRSVEDFQGGNKIEVNKNQFALIEGKNKTVSTLVIQAANVSALYKCEAVNKVGRGERVISFHVTRGPEITLQPDMQPTEQESVSLWCTADRSTFENLTWYKLGPQPLPIHVGELPTPVCKNLDTLWKLNATMFSNSTNDILIMELKNASLQDQGDYVCLAQDRKTKKRHCVVRQLTVLERVAPTITGNLENQTTSIGESIEVSCTASGNPPPQIMWFKDNETLVEDSGIVLKDGNRNLTIRRVRKEDEGLYTCQACSVLGCAKVEAFFIIEGA
• Predicted MW: 116 kDa
• Purity: >95% pure as by SDS-PAGE and silver stain.
• Buffer: Presentation State: Purified
  State: Lyophilized protein
  Buffer System: 25 mM MES, 150 mM NaCl, pH 5.5
  Stabilizer: None
• Bioactivity: Biological: Measured by its ability to inhibit the VEGF165-induced proliferation in Human Umbilical Vein Endothelial (HUVE) cells.
  Inhibition of VEGF165-induced proliferationof HUVECs byrecombinant sVEGFR-2/KDR (Cat.-No DA3530).
• Endotoxin: < 0.1 ng per µg of sKDR
• Reconstitution: The lyophilized Human sKDR is soluble in water and most aqueous buffers.
  Restore in water or PBS to a concentration of not lower than 0.1 mg/ml.
• Preparation: Lyophilized protein
• Protein Description: Recombinant Human soluble Endothelial Growth Factor Receptor-2 (sKDR D1-7) is produced as a non-chimeric protein in a monomeric form.
  The soluble receptor protein consists of all 7 extracellular domains, which contain all the information necessary for high affinity ligand binding.
  The receptor monomers have a mass of approximately 116kDa.
  Result by N-terminal sequencing: ASVGLPSVSL
  Length: 738 amino acids.
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_002244
• Locus ID: 3791
• UniProt ID: P35968
• Cytogenetics: 4q12
• Synonyms: CD309; FLK1; VEGFR; VEGFR2
• Summary: 'Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq, May 2009]'
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Endocytosis, Focal adhesion, VEGF signaling pathway