VEGFR-1 / Flt-1 (D1-D4) Human Protein

CAT#: DA3533X

VEGFR-1 / Flt-1 (D1-D4) human recombinant protein, 20 µg

Product Images

Specifications

Product Data

• Species: Human
• Expression Host: Insect
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  SKLKDPELSLKGTQHIMQAGQTLHLQCRGEAAHKWSLPEMVSKESERLSITKSACGRNGKQFCSTLTLNTAQANHTGFYSCKYLAVPTSKKKETESAIYIFISDTGRPFVEMYSEIPEIIHMTEGRELVIPCRVTSPNITVTLKKFPLDTLIPDGKRIIWDSRKGFIISNATYKEIGLLTCEATVNGHLYKTNYLTHRQTNTIIDVQISTPRPVKLLRGHTLVLNCTATTPLNTRVQMTWSYPDEKNKRASVRRRIDQSNSHANIFYSVLTIDKMQNKDKGLYTCRVRSGPSFKSVNTSVHIYDKAFITVKHRKQQVLETVAGKRSYRLSMKVKAFPSPEVVWLKDGLPATEKSARYLTRGYSLIIKDVTEEDAGNYTILLSIKQSNVFKNLTATLIVNVKPQIYEKAVSSFPDPALYPLGSRQILTCTAYGIPQPTIKWFWHPCNHNHSEARCDFC
• Predicted MW: 55 kDa
• Purity: >90% by SDS-PAGE and visualised by silver stain.
• Buffer: Presentation State: Purified
  State: Lyphilized protein
  Buffer System: PBS
  Stabilizer: None
• Bioactivity: Biological: The activity of sVEGFR-1D1-4 was determined by its ability to inhibit the VEGF-A-induced proliferation of HUVECs.
• Endotoxin: < 0.1 ng per µg of VEGFR-1.
• Reconstitution: The lyophilized sVEGFR-1D1-4 is soluble in water and most aqueous buffers and should be reconstituted in PBS to a concentration not lower than 100 ng/ml.
• Preparation: Lyphilized protein
• Protein Description: Recombinant Human Soluble VEGFR-1 (D1-4).
  Recombinant Human soluble Vascular Endothelial Growth Factor Receptor-1 domain D1-4 (sVEGFR-1D1-4) is produced as a non-chimeric protein in a monomeric form. The soluble receptor protein contains only the first 4 extracellular domains, which contain all the information necessary for binding of VEGF. The receptor monomers have a mass of approximately 55kDa containing 457 amino acid residues.
  Result by N-terminal sequencing: SGSKLKD
• Note: Centrifuge vials before opening!
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_001153392
• Locus ID: 2321
• UniProt ID: P17948
• Cytogenetics: 13q12.3
• Synonyms: FLT; FLT-1; VEGFR-1; VEGFR1
• Summary: 'This gene encodes a member of the vascular endothelial growth factor receptor (VEGFR) family. VEGFR family members are receptor tyrosine kinases (RTKs) which contain an extracellular ligand-binding region with seven immunoglobulin (Ig)-like domains, a transmembrane segment, and a tyrosine kinase (TK) domain within the cytoplasmic domain. This protein binds to VEGFR-A, VEGFR-B and placental growth factor and plays an important role in angiogenesis and vasculogenesis. Expression of this receptor is found in vascular endothelial cells, placental trophoblast cells and peripheral blood monocytes. Multiple transcript variants encoding different isoforms have been found for this gene. Isoforms include a full-length transmembrane receptor isoform and shortened, soluble isoforms. The soluble isoforms are associated with the onset of pre-eclampsia.[provided by RefSeq, May 2009]'
• Protein Families: Druggable Genome, Protein Kinase, Secreted Protein
• Protein Pathways: Cytokine-cytokine receptor interaction, Endocytosis, Focal adhesion