Tumor necrosis factor (TNF-alpha) Canine Protein
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Specifications
Product Data | |
Species | Canine |
Expression Host | Pichia pastoris |
Predicted MW | 17.3 kDa |
Purity | >95% pure by SDS PAGE analysis |
Buffer | Presentation State: Purified State: Lyophilized purified protein Buffer System: PBS. Further dilutions should be made in a buffer containing carrier protein. Preservative: None Stabilizer: None |
Bioactivity | Biological: Has an ED50 of 10pg/ml, determined by measuring the cytolytic effect of recombinant canine TNF alpha on murine WEHI-13VAR-164 cells in the presence of Actinomycin D. |
Endotoxin | < 1.1EU/µg |
Reconstitution | Restore with 0.5 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. We recommend that the vial is gently mixed after reconstitution. |
Preparation | Lyophilized purified protein |
Applications | Functional Assays. |
Protein Description | Recombinant Canine TNF alpha protein expressed in Pichia pastoris. |
Storage | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Reference Data | |
RefSeq | NP_001003244 |
Locus ID | 403922 |
Synonyms | TNFA|cTNF |
Summary | tumor necrosis factor |
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