NEDD4 (NM_006154) Human Recombinant Protein

CAT#: TP321706

Recombinant protein of human neural precursor cell expressed, developmentally down-regulated 4 (NEDD4), transcript variant 1

Product Images

Coomassie blue staining of purified NEDD4 protein (Cat# TP321706). The protein was produced from HEK293T cells transfected with NEDD4 cDNA clone (Cat# RC221706) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Recombinant protein was produced with TrueORF clone, RC221706. Click on the TrueORF clone link to view cDNA and protein sequences.
• Tag: C-Myc/DDK
• Predicted MW: 104 kDa
• Concentration: >50 ug/mL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_006145
• Locus ID: 4734
• UniProt ID: P46934
• Cytogenetics: 15q21.3
• Refseq Size: 5749
• Refseq ORF: 2700
• Synonyms: NEDD4-1; RPF1
• Summary: 'This gene is the founding member of the NEDD4 family of HECT ubiquitin ligases that function in the ubiquitin proteasome system of protein degradation. The encoded protein contains an N-terminal calcium and phospholipid binding C2 domain followed by multiple tryptophan-rich WW domains and, a C-terminal HECT ubiquitin ligase catalytic domain. It plays critical role in the regulation of a number of membrane receptors, endocytic machinery components and the tumor suppressor PTEN. [provided by RefSeq, Jul 2016]'
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis, Ubiquitin mediated proteolysis