RWDD3 (NM_001128142) Human Recombinant Protein
CAT#: TP325219
Recombinant protein of human RWD domain containing 3 (RWDD3), transcript variant 2
View other "RWDD3" proteins (5)
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Specifications
Product Data | |
Species | Human |
Expression Host | HEK293T |
Expression cDNA Clone or AA Sequence |
Recombinant protein was produced with TrueORF clone, RC225219. Click on the TrueORF clone link to view cDNA and protein sequences.
|
Tag | C-Myc/DDK |
Predicted MW | 21.9 kDa |
Concentration | >50 ug/mL as determined by microplate BCA method |
Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
Buffer | 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol |
Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
Storage | Store at -80°C. |
Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
Reference Data | |
RefSeq | NP_001121614 |
Locus ID | 25950 |
UniProt ID | Q9Y3V2, D3DT49 |
Cytogenetics | 1p21.3 |
Refseq ORF | 585 |
Synonyms | RSUME |
Summary | Enhancer of SUMO conjugation. Via its interaction with UBE2I/UBC9, increases SUMO conjugation to proteins by promoting the binding of E1 and E2 enzymes, thioester linkage between SUMO and UBE2I/UBC9 and transfer of SUMO to specific target proteins which include HIF1A, PIAS, NFKBIA, NR3C1 and TOP1. Isoform 1 and isoform 2 positively regulate the NF-kappa-B signaling pathway by enhancing the sumoylation of NF-kappa-B inhibitor alpha (NFKBIA), promoting its stabilization which consequently leads to an increased inhibition of NF-kappa-B transcriptional activity. Isoform 1 and isoform 2 negatively regulate the hypoxia-inducible factor-1 alpha (HIF1A) signaling pathway by increasing the sumoylation of HIF1A, promoting its stabilization, transcriptional activity and the expression of its target gene VEGFA during hypoxia. Isoform 2 promotes the sumoylation and transcriptional activity of the glucocorticoid receptor NR3C1 and enhances the interaction of SUMO1 and NR3C1 with UBE2I/UBC9. Has no effect on ubiquitination. [UniProtKB/Swiss-Prot Function] |
Documents
FAQs |
SDS |
Resources
Recombinant Protein Resources |
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
LC414519 | RWDD3 HEK293T cell transient overexpression lysate (as WB positive control) |
USD 121.00 |
|
LC426894 | RWDD3 HEK293T cell transient overexpression lysate (as WB positive control) |
USD 121.00 |
|
LY414519 | Transient overexpression lysate of RWD domain containing 3 (RWDD3), transcript variant 1 |
USD 396.00 |
|
LY426894 | Transient overexpression lysate of RWD domain containing 3 (RWDD3), transcript variant 2 |
USD 396.00 |
|
PH325219 | RWDD3 MS Standard C13 and N15-labeled recombinant protein (NP_001121614) |
USD 2,055.00 |
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