Dhfr (NM_010049) Mouse Recombinant Protein

CAT#: TP501752

Purified recombinant protein of Mouse dihydrofolate reductase (Dhfr), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Specifications

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  A DNA sequence from Mouse cDNA ORF Clone, MR201752, encoding Mouse full-length Dhfr.
• Tag: C-MYC/DDK
• Predicted MW: 21.6 kDa
• Concentration: >50 ug/mL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_034179
• Locus ID: 13361
• UniProt ID: P00375, Q544T5
• Cytogenetics: 13 47.64 cM
• Refseq Size: 5307
• Refseq ORF: 564
• Synonyms: 8430436I03Rik; AA607882; AI662710; AW555094
• Summary: Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1. [UniProtKB/Swiss-Prot Function]