Kpna2 (BC082280) Mouse Recombinant Protein

CAT#: TP507037

Purified recombinant protein of Mouse karyopherin (importin) alpha 2 (cDNA clone MGC:91246 IMAGE:6334979), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Specifications

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Tag: C-MYC/DDK
• Predicted MW: 48 kDa
• Concentration: >50 ug/mL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Reference Data

• Locus ID: 16647
• Cytogenetics: 11 E1
• Refseq Size: 1866
• Refseq ORF: 1323
• Synonyms: IPOA1
• Summary: Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. [UniProtKB/Swiss-Prot Function]