Akt2 (NM_007434) Mouse Recombinant Protein

CAT#: TP526759

Purified recombinant protein of Mouse thymoma viral proto-oncogene 2 (Akt2), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Specifications

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  A DNA sequence from Mouse cDNA ORF Clone, MR226759, encoding Mouse full-length Akt2.
• Tag: C-MYC/DDK
• Predicted MW: 56.2 kDa
• Concentration: >50 ug/mL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_031460
• Locus ID: 11652
• UniProt ID: Q60823, Q3TY95, Q8CE74
• Cytogenetics: 7 15.94 cM
• Refseq Size: 2957
• Refseq ORF: 1443
• Synonyms: 2410016A19Rik; AW554154; PKB; PKBbeta
• Summary: One of the few specific substrates of AKT2 identified so far is PITX2. Phosphorylation of PITX2 impairs its association with the CCND1 mRNA-stabilizing complex thus shortening the half-life of CCND1. AKT2 seems also to be the principal isoform responsible of the regulation of glucose uptake. Phosphorylates C2CD5 on 'Ser-197' during insulin-stimulated adipocytes. AKT2 is also specifically involved in skeletal muscle differentiation, one of its substrates in this process being ANKRD2. Phosphorylates CLK2 on 'Thr-343'. [UniProtKB/Swiss-Prot Function]