Il1a (NM_010554) Mouse Recombinant Protein

CAT#: TP720032L

Recombinant protein of mouse interleukin-1a/IL-1a

Specifications

Product Data

• Species: Mouse
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Ser115-Ser270
• Tag: tag free
• Predicted MW: 18 kDa
• Concentration: lot specific
• Purity: >95% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: Lyophilized from a 0.2 um filtered solution of 50mM Tris-HCl,200mM NaCl,pH 8.0.
• Bioactivity: ED50 is less than 0.01 ng/ml as determined by the dose-dependent stimulation of mouse D10S cells. Specific Activity of 1.0 x 108 IU/mg.
• Endotoxin: < 0.1 EU per µg protein as determined by LAL test
• Storage: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
• Stability: Stable for at least 6 months from date of receipt under proper storage and handling conditions.

Reference Data

• RefSeq: NP_034684
• Locus ID: 16175
• UniProt ID: P01582, Q3U0Y6
• Cytogenetics: 2 62.9 cM
• Refseq Size: 1974
• Refseq ORF: 813
• Synonyms: Il-1a
• Summary: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.[UniProtKB/Swiss-Prot Function]