NEDD8 (NM_006156) Human Recombinant Protein

CAT#: TP720985M

Purified recombinant protein of Human neural precursor cell expressed, developmentally down-regulated 8 (NEDD8)

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Met1-Gly76
• Tag: N-His&SUMO
• Predicted MW: 20.9 kDa
• Concentration: lot specific
• Purity: >95% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: Lyophilized from a 0.2 um filtered solution of 20mM PB, 150mM NaCl, 5% Trehalose, pH 7.4.
• Endotoxin: Endotoxin level is < 0.1 ng/µg of protein (< 1 EU/µg)
• Storage: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
• Stability: Stable for at least 6 months from date of receipt under proper storage and handling conditions.

Reference Data

• RefSeq: NP_006147
• Locus ID: 4738
• UniProt ID: Q15843
• Cytogenetics: 14q12
• Refseq Size: 625
• Refseq ORF: 243
• Synonyms: NEDD-8
• Summary: Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.[UniProtKB/Swiss-Prot Function]
• Protein Families: Druggable Genome