SECTM1 Human Recombinant Protein

CAT#: TP721363

APC Conjugated Human SECTM1 Protein (C-His)

Specifications

Product Data

• Species: Human
• Expression Host: CHO
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Gln29-Gly145
• Tag: C-His
• Predicted MW: The protein has a predicted molecular weight of 14kDa and migrates at approximately 20kDa on SDS-PAGE with DTT-reduced condition before APC conjugation.
• Concentration: 25µg size is bottled at 0.1mg/mL concentration. 100 µg size is bottled at lot specific concentration.
• Purity: >90%
• Buffer: 1xPBS buffer, pH7.4, 0.09% NaN3 with a carrier protein
• Bioactivity: Positive
  
  The definition of the active protein (purified and biotinylated) is defined as the protein that can bind to its biological receptor/ligand. For conjugated protein, it is defined with its function to bind to the ScFv of the active CAR-transfected cells in flow cytometry test.
• Preparation: Affinity Ni-NTA
• Applications: FACS
• Storage: Unopened vial can be stored at 4°C for six months.
• Stability: 6 Months

Reference Data

• RefSeq: Q8WVN6
• Locus ID: 6398
• UniProt ID: Q8WVN6
• Summary: Human SECTM1 (secreted and transmembrance 1) is a type I transmembrane glycoprotein consisted of a 28 aa signal sequence, a 117 aa extracellular domain (ECD) with one potential N‑linked glycosylation site, a 21 aa transmembrane sequence, and an 82 aa cytoplasmic sequence. It is localized in the Golgi apparatus and exists as a transmembrane and a soluble form. SECTM1 protein expression is detected in some myeloid cells, such as stimulated monocytes, immature monocyte‑derived dendritic cells, and granulocytes. In the thymus, it is also expressed by epithelia and fibroblasts. Its expression is stimulated by IFN-gamma. Although its function is not well understood, the soluble N-terminus form of human SECTM1 demonstrates that it is a costimulatory ligand for human CD4 and CD8 T cell proliferation and cytokine production in CD7-dependent manner.