IL12A (NM_000882) Human Recombinant Protein
CAT#: TP723721
Purified recombinant protein of Human interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35) (IL12A)
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Specifications
Product Data | |
Species | Human |
Expression Host | Sf9 |
Expression cDNA Clone or AA Sequence |
Secreted protein p35: Accession# NM_000882, and p40: Accession# NM_002187
|
Tag | Tag Free |
Predicted MW | 57 kDa |
Concentration | Protein formulated and vialed at >50 ug/mL in specified buffer |
Purity | 95%, as determined by Coomassie stained SDS-PAGE. |
Buffer | 10mM NaH2PO4, 150mM NaCl, pH7.2 |
Bioactivity | The ED50 is 0.05 - 0.25 ng/ml, corresponding to a specific activity of 0.4 - 2.0 x 10^7 units/mg, determined by the production of IFNγ by activated human PBMC in response to IL-12. |
Endotoxin | Less than 0.01 ng per µg protein as determined by the LAL method |
Storage | Store at -80°C. |
Stability | Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
Reference Data | |
RefSeq | NP_000873 |
Locus ID | 3592 |
UniProt ID | P29459, O60595 |
Cytogenetics | 3q25.33 |
Refseq Size | 1450 |
Refseq ORF | 759 |
Synonyms | CLMF; IL-12A; NFSK; NKSF1; P35 |
Summary | 'This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. [provided by RefSeq, Jul 2008]' |
Protein Families | Druggable Genome, Secreted Protein |
Protein Pathways | Allograft rejection, Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway, Type I diabetes mellitus |
Documents
FAQs |
Resources
Recombinant Protein Resources |
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
TP721240 | Purified recombinant protein of Human interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35) (IL12A) |
USD 330.00 |
|
TP723184 | Purified recombinant protein of Human interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35) (IL12A). |
USD 240.00 |
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