TDT (Dntt) (NM_001043228) Mouse Recombinant Protein

CAT#: TP750212

Purified recombinant protein of DNA nucleotidylexotransferase isoform 2, 131Ser-510Ala(336Asn, 454Ala, 457Asn), with N-terminal His tag, expressed in E.coli, 50ug

Product Images

Purified recombinant protein TDT was analyzed by SDS-PAGE gel and Coomossie Blue Staining.

Specifications

Product Data

• Species: Mouse
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  1 MHHHHHHSSP SPVPGSQNVP APAVKKISQY ACQRRTTLNN YNQLFTDALD ILAENDELRE
  61 NEGSCLAFMR ASSVLKSLPF PITSMKDTEG IPCLGDKVKS IIEGIIEDGE SSEAKAVLND
  121 ERYKSFKLFT SVFGVGLKTA EKWFRMGFRT LSKIQSDKSL RFTQMQKAGF LYYEDLVSCV
  181 NRPEAEAVSM LVKEAVVTFL PDALVTMTGG FRNGKMTGHD VDFLITSPEA TEDEEQQLLH
  241 KVTDFWKQQG LLLYCDILES TFEKFKQPSR KVDALDHFQK CFLILKLDHG RVHSEKSGQQ
  301 EGKGWKAIRV DLVMCPYDRR AFALLGWTGS AQFNRDLRRY ATHERKMMLD NHALYDRTKR
  361 VFLEAESEEE IFAHLGLDYI EPWERNA
• Tag: N-His
• Predicted MW: 44.2 kDa
• Concentration: >50 ug/mL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 50mM Tris, pH8.0, 500mM NaCl, 10% Glycerol
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for at least 1 year from receipt of products under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_001036693
• Locus ID: 21673
• UniProt ID: P09838, Q3UZ80
• Cytogenetics: 19 34.32 cM
• Synonyms: Tdt
• Summary: Isoform TDT-S: Transferase that catalyzes the nontemplated addition of nucleoside triphosphate to coding ends during V(D)J recombination (N addition). Involved in the generation of diversity in the antigen-binding region of immunoglobulin heavy and light chains and T-cell receptors during B-and T-cell development. Does not act on double-stranded DNA with blunt ends.[UniProtKB/Swiss-Prot Function]