Rubicon (RUBCN) (NM_014687) Human Recombinant Protein

CAT#: TP762693

Purified recombinant protein of Human KIAA0226 (KIAA0226), transcript variant 2, full length, with N-GST and C-His tag, expressed in E.coli, 50ug

Product Images

Purified recombinant protein KIAA0226 was analyzed by SDS-PAGE gel and Coomossie Blue Staining.

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  A DNA sequence encoding the region full length of KIAA0226
• Tag: N-GST and C-HIS
• Predicted MW: 136.6 kDa
• Concentration: >50 ug/mL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 50mM Tris, pH8.0, 8M Urea
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for at least 1 year from receipt of products under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_055502
• Locus ID: 9711
• UniProt ID: Q92622, Q8N4U6
• Cytogenetics: 3q29
• Refseq Size: 6732
• Refseq ORF: 2916
• Synonyms: KIAA0226; RUBICON; SCAR15
• Summary: The protein encoded by this gene is a negative regulator of autophagy and endocytic trafficking and controls endosome maturation. This protein contains two conserved domains, an N-terminal RUN domain and a C-terminal DUF4206 domain. The RUN domain is involved in Ras-like GTPase signaling, and the DUF4206 domain contains a diacylglycerol (DAG) binding-like motif. Mutation in this gene results in deletion of the DAG binding-like motif and causes a recessive ataxia. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Apr 2014]