pCMV6-AC-FC-S Tagged Cloning Vector

CAT#: PS100054

pCMV6-AC-FC-S, mammalian vector with C-terminal FC and secretion signal, 10ug

Product Images

Specifications

Product Data

• sds_url: /sds/ps100054.pdf
• expression: Mammalian
• sub_category: Tagged Cloning Vectors
• ecoli_selection: Ampicillin
• availabilities_filter: In Stock
• multiple_cloning: /assets/images/trueorf/ps100054-mcs.gif
• expression_promoter: CMV
• description: pCMV6-AC-FC-S, mammalian vector with C-terminal FC and secretion signal, 10ug
• product_name: pCMV6-AC-FC-S Tagged Cloning Vector
• features:
  • ORFs cloned in this vector will be expressed in mammalian cells as a secreted tagged protein with a C-terminal murine Fc tag. Due to the presence of an N-terminal secretion signal sequence, the fusion protein will be secreted and this facilitates the downstream purification
  • The Fc-tag encodes the CH2 and CH3 domains of the mouse IgG heavy chain and the hinge region. A fusion protein is comprised of two relatively independent parts connected with a flexible spacer. Fc fusion provides several advantages in protein applications:
    1. Easy purification using protein A or protein G affinity chromatography.
    2. Increased solubility.
    3. Excellent antigen in mouse mAB production without cleavage, as the murine Fc is not antigenic in mice.
  • If the C-terminal tagging interferes with the protein’s function, you can choose the N-terminal Fc-tagging vector, PS100056
  • Validation Data
  • Download full vector sequence
• cell_selection: Neomycin
• citations: Yes
• terms: PCMV
• name: pCMV6-AC-FC-S
• tagged_vector_tab: epitope
• ctag: FC
• vector: Destination Vector
• category: Vectors
• sizes_filter: 10 ug