pCMV6-Entry2 Tagged Cloning Vector

CAT#: PS100063

pCMV6-Entry2, mammalian vector with C-terminal Myc-DDK tag, 10ug

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Specifications

Product Data

• sds_url: /sds/ps100063.pdf
• expression: Mammalian
• sub_category: Tagged Cloning Vectors
• ecoli_selection: Kanamycin
• availabilities_filter: In Stock
• multiple_cloning: /assets/images/trueorf/ps100063-mcs.gif
• expression_promoter: CMV
• description: pCMV6-Entry2, mammalian vector with C-terminal Myc-DDK tag, 10ug
• product_name: pCMV6-Entry2 Tagged Cloning Vector
• features:
  • pCMV6-Entry2 vector is the secondary entry vector for TrueORF clones, This vector is used when the insert is difficult to be cloned into the primary entry vector, pCMV6-Entry.
  • pCMV6-Entry2 vector exhibits the same functional properties of pCMV6-Entry, including kanamycin-resistance in E.coli, Neomycin-resistance in mammalian cells, protein expression driven by CMV. The multiple cloning sites are also identical to pCMV6-Entry.
  • ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with the C-terminal Myc-DDK tags. (DDK is the same as FLAG® which is a registered trademark of Sigma Aldrich).
  • Such clones are the best for detection and purification of the transgene using anti-Myc or anti-DDK antibodies.
  • Serve as the entry vector in the PrecisionShuttle system to transfer the ORF sequence into any destination vectors for other tagging options or other expression platforms.
  • Download full vector sequence
• cell_selection: Neomycin
• terms: PCMV
• name: pCMV6-Entry2
• ctag: Myc-DDK
• vector: Destination Vector
• category: Vectors
• sizes_filter: 10 ug