pEX-C-His Tagged Cloning Vector

CAT#: PS100076

pEX-C-His, bacterial expression vector with C-terminal His tag, 10ug

Product Images

Specifications

Product Data

• sds_url: /sds/ps100076.pdf
• expression: Escherichia coli
• sub_category: Tagged Cloning Vectors
• ecoli_selection: Ampicillin
• availabilities_filter: In Stock
• multiple_cloning: /assets/images/trueorf/ps100076-mcs.gif
• description: pEX-C-His, bacterial expression vector with C-terminal His tag, 10ug
• product_name: pEX-C-His Tagged Cloning Vector
• features:

  This vector is for E.coli expression.

  • Upon IPTG induction, ORFs cloned in this vector can be expressed in E.coli (BL21/DE3) as tagged protein with a C-terminal His tag.
  • Unlike other PrecisionShuttle vectors, this vector does not have an Sgf I site at the multiple cloning site. Instead a BseR I site can be used to generate Sgf I-compatible end for in-frame shuttling from TrueORF clones in pEntry vector.
  • Detection and purification of the transgene are best done using an anti-His antibody or a nickel affinity column.
  • If the C-terminal tagging interferes with the protein’s function, you can choose the N-terminal His-tagging vector, PS100030
  • Download full vector sequence
• citations: Yes
• name: pEX-C-His
• tagged_vector_tab: bacterial
• ctag: His
• vector: Destination Vector
• category: Vectors
• sizes_filter: 10 ug