Secondary Antibodies

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Mouse IgG (H+L chain) sheep polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation FITC
Immunogen Mouse IgG whole molecule.

Guinea Pig IgG (Fc specific) sheep polyclonal antibody, FITC

Applications ELISA.
(In)direct immunofluorescence.

Immunocytochemistry.
Immunohistochemistry on Frozen Sections.
Can be  used in direct staining of cytoplasmic IgG in fixed guinea pig cells and tissue substrates; to identify circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of guinea pig in the middle layer of the test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions: 1/20-1/80.
Reactivities Guinea Pig
Conjugation FITC
Immunogen Purified normal IgG isolated from pooled guinea pig serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Bovine IgG sheep polyclonal antibody, FITC

Applications Flow Cytometry.
Immunohistochemistry on Frozen Sections: 1/200-1/2,000.
Reactivities Bovine
Conjugation FITC
Immunogen Purified Bovine IgG.

Bovine IgM sheep polyclonal antibody, FITC

Applications Flow Cytometry: 1/20-1/100.
Immunohistochemistry on frozen sections: 1/200-1/2000.
Reactivities Bovine
Conjugation FITC
Immunogen Purified Bovine IgM

Bovine IgG1 sheep polyclonal antibody, FITC

Applications Flow Cytometry: 1/20 - 1/100.
Immunohistochemistry on frozen sections: 1/20 - 1/100.
Reactivities Bovine
Conjugation FITC
Immunogen Purified bovine IgG1

Bovine IgG2 sheep polyclonal antibody, FITC

Applications Flow Cytometry: 1/20 - 1/100.
Immunohistochemistry on frozen sections: 1/20 - 1/100.
Reactivities Bovine
Conjugation FITC
Immunogen Purified bovine IgG2

Rabbit IgG (H+L chain) sheep polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation FITC
Immunogen Rabbit IgG whole molecule.

Rabbit IgG (H+L chain) sheep polyclonal antibody, FITC

Applications Flow Cyometry: 1/25 - 1/50; Use 50µl of the suggested working dilution to label 10e6 cells in 100µl.
Immunohistochemistry on frozen sections: 1/25 - 1/50.
Conjugation FITC
Immunogen Rabbit IgG