Secondary Antibodies

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Human IgG3 sheep polyclonal antibody, HRP

Applications ELISA.
Immunohistochemistry on Frozen Sections: 1/100-1/200.
Reactivities Human
Conjugation HRP
Immunogen Human IgG3 (kappa & lambda) purified from Human myeloma serum

Bovine IgG1 sheep polyclonal antibody, AP

Applications ELISA: 1/1000-1/10000.
Reactivities Bovine
Conjugation AP
Immunogen Purified Bovine IgG1.

Human IgA (Fc specific) sheep polyclonal antibody, TRITC

Applications Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of IgA. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity. This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected.
Recommended Dilutions: 1/10-1/40
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Reactivities Human
Conjugation TRITC
Immunogen Purified IgA prepared from pooled Human serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Human IgG (Fc specific) sheep polyclonal antibody, TRITC

Applications Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of IgG. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity. This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected.
Recommended Dilutions: 1/10-1/40
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Reactivities Human
Conjugation TRITC
Immunogen Purified IgG prepared from pooled Human serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Rabbit IgG sheep polyclonal antibody, Purified

Applications ELISA.
Western Blot: 1/1000.
Immunohistochemistry on Frozen Sections.
Reactivities Rabbit
Immunogen Purified Rabbit IgG

Bovine IgG2 sheep polyclonal antibody, AP

Applications ELISA: 1/1000-1/10000.
Reactivities Bovine
Conjugation AP
Immunogen Purified Bovine IgG2.

Human IgG1 sheep polyclonal antibody, Purified

Applications ELISA: 1/4000-1/8000
Western Blot: 1/4000-1/8000
Reactivities Human
Immunogen Purified Human IgG1.

Bovine IgG sheep polyclonal antibody, AP

Applications

ELISA: 1/5000-1/50000.
Western Blot: 1/5000-1/50000.

Reactivities Bovine
Conjugation AP
Immunogen Purified Bovine IgG.

Bovine IgG sheep polyclonal antibody, FITC

Applications Flow Cytometry.
Immunohistochemistry on Frozen Sections: 1/200-1/2,000.
Reactivities Bovine
Conjugation FITC
Immunogen Purified Bovine IgG.

Bovine IgG sheep polyclonal antibody, HRP

Applications ELISA: 1/10,000-1/100,000. 
Western Blot.
 
Reactivities Bovine
Conjugation HRP
Immunogen Purified Bovine IgG.

Bovine IgG sheep polyclonal antibody, Purified

Applications ELISA: 1/100-1/10,000.
Immunodiffusion.
Reactivities Bovine
Immunogen Purified Bovine IgG.

Bovine IgM sheep polyclonal antibody, AP

Applications ELISA: 1/1000-1/10000. 
Western Blot: 1/5000-1/50000.
Reactivities Bovine
Conjugation AP
Immunogen Purified Bovine IgM.