Secondary Antibodies

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Human IgM (Mu chain, Fc-Region) (in vitro) mouse monoclonal antibody, clone 2B9

Reactivities Human

Rat IgG2b (Gamma-2b chain specific) mouse monoclonal antibody, clone 2B 10A8, Purified

Applications Flow Cytometry.
Immunofluorescence Microscopy: Identification and enumeration of IgG2b+ cells.
ELISA.
Second step reagent for rat IgG2b Monoclonal Antibodies.
Reactivities Rat

Chicken IgA (alpha chain specific) mouse monoclonal antibody, clone A-1, Purified

Applications ELISA.
Flow Cytometry.
Immunofluorescence.
Immunohistochemistry on Frozen Sections.
Reactivities Chicken

Human IgG (Fc specific) mouse monoclonal antibody, clone H2, Low Endotoxin

Applications Indirect Immunofluorescent staining of IgG+ human B lymphocytes.
ELISA.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunohistochemistry on Frozen Sections.
Immunocytochemistry.
Reactivities Human

Human IgG (Fc specific) mouse monoclonal antibody, clone H2, Purified

Applications Indirect Immunofluorescent staining of IgG+ human B lymphocytes: ≤ 1 µg/106 cells.
ELISA.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunohistochemistry on Frozen Sections.
Immunocytochemistry.
Reactivities Human

Human IgE mouse monoclonal antibody, clone 904, Purified

Applications ELISA.
Reactivities Human

Human IgG (F(ab)2 specific) mouse monoclonal antibody, clone 4A11, Purified

Applications ELISA.
Western blotting.
Flow Cytometry.

Has been described for previous lots to work in Immunofluorescence.
Reactivities Human

Human IgM (Mu chain, Fab-Region) mouse monoclonal antibody, clone MA2

Reactivities Human

Porcine IgM mouse monoclonal antibody, clone 206-2, Aff - Purified

Applications ELISA: 0.1 µg/ml (1/4000).
Immunoprecipitation.
Immunohistochemistry on Frozen Sections: 0.2 µg/ml (1/2000).
Immunohistochemistry on Paraffin Sections (Does not react on routinely processed Paraffin Section).
Does not react in Western Blot with reduced and denatured samples.
Reactivities Porcine

Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 1C5, Biotin

Applications Flow Cytometry: < / = 0.3 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells.
ELISA: Can be used as Capture antibody.
Reactivities Rat
Conjugation Biotin

Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 1C5, Purified

Applications Flow Cytometry.
Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells.
ELISA: Can be used as Capture antibody.
Second step reagent for rat IgG1 Monoclonal Antibodies.
Reactivities Rat

Chicken IgA (alpha chain specific) mouse monoclonal antibody, clone A-1, Biotin

Applications ELISA.
Flow Cytometry: < / = 1 µg/10e6 cells.
Immunofluorescence.
Conjugation Biotin

Human IgG (Fc specific) mouse monoclonal antibody, clone H2, Biotin

Applications Indirect Immunofluorescent staining of IgG+ human B lymphocytes: < / = 1 µg/10e6 cells.
ELISA: 1/10,000-1/20,000.
Reactivities Human
Conjugation Biotin

Human IgG (Fc specific) mouse monoclonal antibody, clone H2, HRP

Applications ELISA: 1/4,000-1/8,000 (See also Ref.1).
Reactivities Human
Conjugation HRP

Rat IgG Light Chain mouse monoclonal antibody, clone KT100, HRP

Applications ELISA: 1/3000.
Western blot: 1/1000 (DAB as substrate) and 1/10000 (ECL system).
Reactivities Rat
Conjugation HRP

Rat IgG Light Chain mouse monoclonal antibody, clone KT100, HRP

Applications ELISA: 1/3000.
Western blot: 1/1000 (DAB as substrate) and 1/10000 (ECL system).
Reactivities Rat
Conjugation HRP

Rat IgG Light Chain mouse monoclonal antibody, clone KT100, HRP

Applications ELISA: 1/3000.
Western blot: 1/1000 (DAB as substrate) and 1/10000 (ECL system).
Reactivities Rat
Conjugation HRP

Rat IgG Light Chain mouse monoclonal antibody, clone KT100, Aff - Purified

Applications ELISA: 1/3000.
Western Blot: 1/1000 dilution using DAB as substrate, 1/10000 dilution using ECL system.
Reactivities Rat

Rat IgG Light Chain mouse monoclonal antibody, clone KT100, Aff - Purified

Applications ELISA: 1/3000.
Western Blot: 1/1000 dilution using DAB as substrate, 1/10000 dilution using ECL system.
Reactivities Rat

Rat IgG Light Chain mouse monoclonal antibody, clone KT100, Aff - Purified

Applications ELISA: 1/3000.
Western Blot: 1/1000 dilution using DAB as substrate, 1/10000 dilution using ECL system.
Reactivities Rat

Human IgE mouse monoclonal antibody, clone ME.114, Purified

Applications ELISA.
Reactivities Human

Human IgG (Fc specific) mouse monoclonal antibody, clone H2, AP

Applications ELISA: 1/2000-1/8,000.
Reactivities Human
Conjugation AP

Human IgM (Mu chain, Fab-Region) mouse monoclonal antibody, clone B1545M, Purified

Applications
This antibody can pair with itself in ELISA, EIA.
Can be used for specific IgM antibodies detection and for total IgM level detection.
Can also be used as a capture antibody for total IgM level detection.
Reactivities Human