Secondary Antibodies

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Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, PE

Applications FLISA: 1 µg/ml.
Immunocytochemistry.
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation PE

Human IgG (Fc specific) mouse monoclonal antibody, clone MK1A6, Purified

Applications ELISA assay development of total IgG.
Passive hemagglutination assays.
Histological staining of Acetone Fixed Frozen tissue sections.
Reactivities Human

Human IgM mouse monoclonal antibody, clone ICL-931, FITC

Applications ELISA.
Immunohistochemistry on Frozen Sections: 1/40. 
Staining of Human cells and tissue sections.
Reactivities Human
Conjugation FITC

Human IgG mouse monoclonal antibody, clone EFE-565, FITC

Applications ELISA.
Immunohistochemistry on frozen sections: 1/40.
Reactivities Human
Conjugation FITC

Human IgA mouse monoclonal antibody, clone BFG-267, FITC

Applications ELISA.
Immunohistochemistry on frozen sections: 1/40.
Reactivities Human
Conjugation FITC

Rat IgM (Mu chain specific) mouse monoclonal antibody, clone HIS40, Supernatant

Applications

Flow cytometry.
Immunofluorescence.
Immunohistochemistry on frozen tissue sections.

Reactivities Rat

Human IgA (Secretory component) mouse monoclonal antibody, clone MC29/12, Purified

Applications Immunohistochemistry on frozen and paraffin sections.
ELISA.
Reactivities Human

Mouse IgG1 (heavy chain) rat monoclonal antibody, clone LO-MG1-2, Purified

Applications Useful as second antibody in immunoassays, such as ELISA's (good binding on plastic), Immunohistochemistry on Frozen Sections and for Affinity Chromatography of murine IgG1.
Reactivities Mouse

Mouse IgE (heavy chain) rat monoclonal antibody, clone LO-ME-3, Biotin

Applications Useful as second antibody in Immunoassays, such as ELISA's (good binding on plastic) and Immunohistochemistry on Frozen Sections.
Reactivities Mouse
Conjugation Biotin

Mouse IgE (heavy chain) rat monoclonal antibody, clone LO-ME-3, FITC

Applications Flow Cytometry: Use 50µl of 5µg/ml diluted antibody to label 10e6 cells in 100µl.
Reactivities Mouse
Conjugation FITC

Mouse IgE (heavy chain) rat monoclonal antibody, clone LO-ME-3, HRP

Applications Useful as second antibody in Immunoassays, such as ELISA's (good binding on plastic) and Immunohistochemistry on Frozen Sections.
Reactivities Mouse
Conjugation HRP

Mouse IgE (heavy chain) rat monoclonal antibody, clone LO-ME-3, Purified

Applications Useful as second antibody in Immunoassays, such as ELISA's (good binding on plastic) and Immunohistochemistry on Frozen Sections.
Reactivities Mouse

Rat IgG2b (heavy chain) mouse monoclonal antibody, clone MARG2b-3, FITC

Applications Flow Cytometry: 5 µg/ml, use 50 µl of the suggested working dilution to label 10e6 cells in 100µl.
Reactivities Rat
Conjugation FITC

Rabbit IgM (B Cell Marker) mouse monoclonal antibody, clone NRBM, Purified

Applications Immunohistochemistry on Frozen Sections.
Flow Cytometry:
Use 10 µl of diluted antibody to label 1x10e6 cells in 100 µl.
Reactivities Rabbit

Human IgA (alpha chain) mouse monoclonal antibody, clone AD3, Purified

Applications ELISA.
Immunohistochemistry on Paraffin Sections: 10 μg/ml.
Positive tissue: colon.
Reactivities Human

Human IgG4 mouse monoclonal antibody, clone MRQ-44, Purified

Applications Immunohistochemistry on Frozen and Formalin-Fixed, Paraffin-Embedded Sections: 1/100-1/500. 
Preparation and Pretreatment:
1. Cut 3-4 μm section of formalin-fixed paraffin-embedded tissue and place on positively charged slides; dry overnight at 58°C.
2. Deparaffinize, rehydrate, and epitope retrieve; the preferred method is the use of Heat Induced Epitope Retrieval (HIER) techniques in conjunction with a pressure cooker. The preferred method allows for simultaneous deparaffinization, rehydration, and epitope retrieval. Upon completion, rinse with 5 changes of distilled or deionized water.
3. If using HRP detection system, place slides in peroxide block for 10 minutes; rinse. If using AP detection system, omit this step.
Positive Control: Tonsil.
Reactivities Human

Human IgA mouse monoclonal antibody, clone DA43D3, Purified

Applications Suitable for Immunohistochemistry on Frozen (1 µg/ml, 1/400) and Paraffin Embedded Sections. Proteinase K pretreatment for antigen retrieval is recommended.
Suggested Positive Control: Human tonsil.
Reactivities Human

Human IgM mouse monoclonal antibody, clone DM3A3, Purified

Applications Immunohistochemistry on frozen sections: 1-2 µg/ml (1:200 - 1:400).
Immunohistochemistry on paraffin sections: Proteinase K pretreatment for antigen retrieval is recommended.
Suggested positive control: Human tonsil.
Reactivities Human

Human IgD (Fc specific) mouse monoclonal antibody, clone LE2, Ig Fraction

Applications Flow cytometry.
Immunohistochemistry on frozen tissue sections.
Reactivities Human