Secondary Antibodies

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Feline IgM goat polyclonal antibody, FITC

Applications Flow Cytometry.
Immunohistochemistry on Frozen Sections.
Reactivities Feline
Conjugation FITC

Porcine IgG F(c) specific goat polyclonal antibody, FITC

Applications Flow Cytometry: 1/20-1/100.
Immunohistochemistry on Frozen Sections: 1/20-1/100.
Reactivities Porcine
Conjugation FITC

Chicken IgM goat polyclonal antibody, FITC

Applications Flow Cytometry.
Immunohistochemistry on frozen sections: 1/200 - 1/2,000.
Reactivities Chicken
Conjugation FITC

Chicken IgA goat polyclonal antibody, FITC

Applications Flow Cytometry.
Immunohistochemistry on frozen sections: 1/200 - 1/2,000.
Reactivities Chicken
Conjugation FITC

Chicken IgG / Chicken IgY (Fc specific) goat polyclonal antibody, FITC

Applications Flow cytometry.
Reactivities Chicken
Conjugation FITC

Rabbit IgG (H+L chain) sheep polyclonal antibody, Biotin

Applications ELISA: 1/25600 - 1/51200.
Western Blot: 1/20000.
Immunohistochemistry on frozen sections: 1/400 - 1/800.
Immunohistochemistry on paraffin sections: 1/100 - 1/200.
Reactivities Rabbit
Conjugation Biotin

Guinea pig IgG (H+L chain) goat polyclonal antibody, FITC

Applications Flow Cytometry.
Immunohistochemistry on frozen sections: 1:200.
Reactivities Guinea Pig
Conjugation FITC

Guinea pig IgG (H+L chain) goat polyclonal antibody, HRP, Purified

Applications ELISA (1/1000-1/10,000).
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Sections.
Reactivities Guinea Pig
Conjugation HRP

Porcine IgG (H+L chain) goat polyclonal antibody, FITC

Applications Flow Cytometry.
Immunohistochemistry on frozen sections: 1:200 - 1:2000.
Reactivities Porcine
Conjugation FITC

Human IgG4 mouse monoclonal antibody, clone MRQ-44, Purified

Applications Immunohistochemistry on Frozen and Formalin-Fixed, Paraffin-Embedded Sections: 1/100-1/500. 
Preparation and Pretreatment:
1. Cut 3-4 μm section of formalin-fixed paraffin-embedded tissue and place on positively charged slides; dry overnight at 58°C.
2. Deparaffinize, rehydrate, and epitope retrieve; the preferred method is the use of Heat Induced Epitope Retrieval (HIER) techniques in conjunction with a pressure cooker. The preferred method allows for simultaneous deparaffinization, rehydration, and epitope retrieval. Upon completion, rinse with 5 changes of distilled or deionized water.
3. If using HRP detection system, place slides in peroxide block for 10 minutes; rinse. If using AP detection system, omit this step.
Positive Control: Tonsil.
Reactivities Human

Sheep/Goat IgG donkey polyclonal antibody, FITC

Applications Immunohistochemistry on Frozen Sections: 1/100-1/200.
Reactivities Goat, Sheep
Conjugation FITC

Human IgD rabbit polyclonal antibody, Purified

Applications Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/50-1/200
Pretreatment of deperaffinized tissue with heat-induced epitope retrieval is recommended.
Use Polymer anti Mouse/Rabbit immunoglobulin as a detection system.
Positive Control: Lymph node.
Reactivities Human

Human IgA rabbit polyclonal antibody, Purified

Applications Immunohistochemistry on Paraffin Sections: 5 µg/ml.
Reactivities Human

Human IgA mouse monoclonal antibody, clone DA43D3, Purified

Applications Suitable for Immunohistochemistry on Frozen (1 µg/ml, 1/400) and Paraffin Embedded Sections. Proteinase K pretreatment for antigen retrieval is recommended.
Suggested Positive Control: Human tonsil.
Reactivities Human

Human IgM mouse monoclonal antibody, clone DM3A3, Purified

Applications Immunohistochemistry on frozen sections: 1-2 µg/ml (1:200 - 1:400).
Immunohistochemistry on paraffin sections: Proteinase K pretreatment for antigen retrieval is recommended.
Suggested positive control: Human tonsil.
Reactivities Human

Human IgD (Fc specific) mouse monoclonal antibody, clone LE2, Ig Fraction

Applications Flow cytometry.
Immunohistochemistry on frozen tissue sections.
Reactivities Human

Human IgD (Delta chain specific) rabbit polyclonal antibody, Purified

Applications Immunohistochemistry on paraffin embedded sections:
diluted of 1:40-1:100 in an ABC method (30-60 minutes at room temperature)
Recommended Positive Control: Tonsil
Reactivities Human

Human IgD (Delta chain specific) rabbit polyclonal antibody, Purified

Applications Immunohistochemistry on paraffin embedded sections:
diluted of 1:40-1:100 in an ABC method (30-60 minutes at room temperature)
Recommended Positive Control: Tonsil
Reactivities Human