Secondary Antibodies

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Human IgM (heavy chain) mouse monoclonal antibody, clone IM373, Purified

Applications ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunofluorescence: 0.5-1 μg/ml.
Western Blot: 0.5-1 μg/ml.
Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
Immunohistochemistry on Frozen and Formalin-Fixed Sections: 0.5-1 μg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 
Recommended Positive Control
: 293T, Raji or hPBL cells, Tonsil or Spleen.
Reactivities Human

Human IgM (heavy chain) mouse monoclonal antibody, clone IM373, Purified

Applications ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunofluorescence: 0.5-1 μg/ml.
Western Blot: 0.5-1 μg/ml.
Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
Immunohistochemistry on Frozen and Formalin-Fixed Sections: 0.5-1 μg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 
Recommended Positive Control
: 293T, Raji or hPBL cells, Tonsil or Spleen.
Reactivities Human

Human IgM (Mu chain, Fc-Region) (in vitro) mouse monoclonal antibody, clone 2B9

Reactivities Human

Human IgG (Fc specific) mouse monoclonal antibody, clone H2, Low Endotoxin

Applications Indirect Immunofluorescent staining of IgG+ human B lymphocytes.
ELISA.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunohistochemistry on Frozen Sections.
Immunocytochemistry.
Reactivities Human

Human IgG (Fc specific) mouse monoclonal antibody, clone H2, Purified

Applications Indirect Immunofluorescent staining of IgG+ human B lymphocytes: ≤ 1 µg/106 cells.
ELISA.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunohistochemistry on Frozen Sections.
Immunocytochemistry.
Reactivities Human

Human IgG (F(ab)2 specific) mouse monoclonal antibody, clone 4A11, Purified

Applications ELISA.
Western blotting.
Flow Cytometry.

Has been described for previous lots to work in Immunofluorescence.
Reactivities Human

Rat IgG Light Chain mouse monoclonal antibody, clone KT100, HRP

Applications ELISA: 1/3000.
Western blot: 1/1000 (DAB as substrate) and 1/10000 (ECL system).
Reactivities Rat
Conjugation HRP

Rat IgG Light Chain mouse monoclonal antibody, clone KT100, HRP

Applications ELISA: 1/3000.
Western blot: 1/1000 (DAB as substrate) and 1/10000 (ECL system).
Reactivities Rat
Conjugation HRP

Rat IgG Light Chain mouse monoclonal antibody, clone KT100, HRP

Applications ELISA: 1/3000.
Western blot: 1/1000 (DAB as substrate) and 1/10000 (ECL system).
Reactivities Rat
Conjugation HRP

Rat IgG Light Chain mouse monoclonal antibody, clone KT100, Aff - Purified

Applications ELISA: 1/3000.
Western Blot: 1/1000 dilution using DAB as substrate, 1/10000 dilution using ECL system.
Reactivities Rat

Rat IgG Light Chain mouse monoclonal antibody, clone KT100, Aff - Purified

Applications ELISA: 1/3000.
Western Blot: 1/1000 dilution using DAB as substrate, 1/10000 dilution using ECL system.
Reactivities Rat

Rat IgG Light Chain mouse monoclonal antibody, clone KT100, Aff - Purified

Applications ELISA: 1/3000.
Western Blot: 1/1000 dilution using DAB as substrate, 1/10000 dilution using ECL system.
Reactivities Rat