Secondary Antibodies

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Peroxidase Conjugated Affinity Purified Goat anti-Mouse IgG [H&L]

Applications WB: 1: 1,000; ICC: 1:200; IP: 1:200
Reactivities Mouse IgG
Conjugation HRP

FITC Conjugated Goat Anti-Rabbit IgG

Applications FC: 1:100; IF/ICC: 1:32-64; IHC: 1:32-64
Reactivities Rabbit IgG
Conjugation FITC

Rabbit Anti-Mouse IgG Secondary Antibody

Applications WB, IHC, IF/ICC, ELISA
Reactivities Mouse IgG
Conjugation Unconjugated

FITC Conjugated Goat Anti-Rabbit IgG

Applications FC: 1:100; IF/ICC: 1:32-64; IHC: 1:32-64
Reactivities Rabbit IgG
Conjugation FITC

Rabbit IgG (H+L chain) goat polyclonal antibody, Aff - Purified

Applications Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions
IF Microscopy.
ELISA: 1/10,000-1/60,000.
Western blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500-1/2500.
Immunoprecipitation.
Reactivities Rabbit

Human IgE goat polyclonal antibody, HRP

Applications ELISA: 1/10000-1/100000.
Reactivities Human, Monkey
Conjugation HRP

FITC Conjugated Goat Anti-Mouse IgG

Applications FC: 1:100; IF/ICC: 1:32-64; IHC: 1:32-64
Reactivities Mouse IgG
Conjugation FITC

Monkey IgG (Fab specific) rabbit polyclonal antibody, FITC

Applications Direct staining of fixed cell and tissue substrates; to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa or lambda type. In general this conjugate is not recommended as direct or indirect screening reagent for If isotypes on surface membranes of vital lymphoid cells. The activity to the common Ig/Fab subunit may result in the staining of immunoglobulins bound to the Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1:20 and 1:80.
Reactivities Monkey
Conjugation FITC

Peroxidase Conjugated Goat Anti-Mouse IgM (u-chain specific)

Applications WB: 1:3,000-10,000; ELISA: 1:20,000-40,000
Reactivities Mouse IgM
Conjugation HRP

Bovine IgG1 sheep polyclonal antibody, AP

Applications ELISA: 1/1000-1/10000.
Reactivities Bovine
Conjugation AP

Goat Polyclonal Anti-Human IgG Antibody

Applications WB 1:500-1:2,000
Reactivities Human

FITC Conjugated Goat Anti-human IgA

Applications FC: 1:100; IF/ICC: 1:32-64; IHC: 1:32-64
Reactivities Human IgA
Conjugation FITC