Secondary Antibodies

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Human IgG4 mouse monoclonal antibody, clone MRQ-44, Purified

Applications Immunohistochemistry on Frozen and Formalin-Fixed, Paraffin-Embedded Sections: 1/100-1/500. 
Preparation and Pretreatment:
1. Cut 3-4 μm section of formalin-fixed paraffin-embedded tissue and place on positively charged slides; dry overnight at 58°C.
2. Deparaffinize, rehydrate, and epitope retrieve; the preferred method is the use of Heat Induced Epitope Retrieval (HIER) techniques in conjunction with a pressure cooker. The preferred method allows for simultaneous deparaffinization, rehydration, and epitope retrieval. Upon completion, rinse with 5 changes of distilled or deionized water.
3. If using HRP detection system, place slides in peroxide block for 10 minutes; rinse. If using AP detection system, omit this step.
Positive Control: Tonsil.
Reactivities Human

Human IgA mouse monoclonal antibody, clone DA43D3, Purified

Applications Suitable for Immunohistochemistry on Frozen (1 µg/ml, 1/400) and Paraffin Embedded Sections. Proteinase K pretreatment for antigen retrieval is recommended.
Suggested Positive Control: Human tonsil.
Reactivities Human

Human IgM mouse monoclonal antibody, clone DM3A3, Purified

Applications Immunohistochemistry on frozen sections: 1-2 µg/ml (1:200 - 1:400).
Immunohistochemistry on paraffin sections: Proteinase K pretreatment for antigen retrieval is recommended.
Suggested positive control: Human tonsil.
Reactivities Human

Human IgA (Fc specific) mouse monoclonal antibody, clone 15D6, Purified

Applications ELISA: 1/500-1/1000.
Reactivities Human

Human IgA1 (Fc specific) mouse monoclonal antibody, clone 3C10, Purified

Applications ELISA.
Reactivities Human

Human IgA2 (Fc specific) mouse monoclonal antibody, clone 1A4, Purified

Applications ELISA.
Reactivities Human

Human IgD (Fc specific) mouse monoclonal antibody, clone LE2, Ig Fraction

Applications Flow cytometry.
Immunohistochemistry on frozen tissue sections.
Reactivities Human

Human IgM mouse monoclonal antibody, clone M15/8, FITC

Applications Flow cytometry.
Reactivities Human
Conjugation FITC

Mouse IgG1 (heavy chain) rat monoclonal antibody, clone LO-MG1-2, Biotin

Applications ELISA: 500ng/ml.
Reactivities Mouse
Conjugation Biotin

Mouse IgG1 (heavy chain) rat monoclonal antibody, clone LO-MG1-2, Purified

Applications ELISA: 5 µg/ml as coating antibody.
Reactivities Mouse

Mouse IgG2a (heavy chain) rat monoclonal antibody, clone LO-MG2a-7, Biotin

Applications ELISA: 0.5µg/ml - 1.0µg/ml.
Reactivities Mouse
Conjugation Biotin

Mouse IgG2a (heavy chain) rat monoclonal antibody, clone LO-MG2a-7, HRP

Applications ELISA: 0.5 µg/ml as coating antibody.
Reactivities Mouse
Conjugation HRP

Mouse IgG2b (heavy chain) rat monoclonal antibody, clone LO-MG2b-2, Biotin

Applications ELISA: 500 ng/ml as a detection antibody.
Reactivities Mouse
Conjugation Biotin

Mouse IgG2b (heavy chain) rat monoclonal antibody, clone LO-MG2b-2, FITC

Applications Flow Cytometry: Use 10 µl of 5 µg/ml diluted antibody to label 106 cells in 100 µl.
Reactivities Mouse
Conjugation FITC

Mouse IgG2b (heavy chain) rat monoclonal antibody, clone LO-MG2b-2, Purified

Applications ELISA: 1.25 µg/ml as a detection antibody.
Reactivities Mouse

Mouse IgE (heavy chain) rat monoclonal antibody, clone LO-ME-3, Biotin

Applications ELISA (Use at  0.5 µg/ml as a detection antibody, avidity 3 x 10e9 M-1).
Reactivities Mouse
Conjugation Biotin

Mouse IgE (heavy chain) rat monoclonal antibody, clone LO-ME-3, FITC

Applications Useful as second antibody in Immunoassays, such as ELISA's
Reactivities Mouse
Conjugation FITC

Mouse IgE (heavy chain) rat monoclonal antibody, clone LO-ME-3, HRP

Applications ELISA: 500ng/ml.
Reactivities Mouse
Conjugation HRP

Mouse IgE (heavy chain) rat monoclonal antibody, clone LO-ME-3, Purified

Applications ELISA: 5 µg/ml as capture antibody.
Reactivities Mouse

Rat IgA (heavy chain) mouse monoclonal antibody, clone MARA-1, Biotin

Applications ELISA: 500 ng/ml.
Reactivities Rat
Conjugation Biotin

Rat IgA (heavy chain) mouse monoclonal antibody, clone MARA-1, FITC

Applications Flow cytometry (5 µg/ml): use 50 µl of the suggested working dilution to label
10e6 cells in 100 µl.
Reactivities Rat
Conjugation FITC

Rat IgD (heavy chain) mouse monoclonal antibody, clone MARD-3, Biotin

Applications ELISA: 500 ng/ml.
Reactivities Rat
Conjugation Biotin

Rat IgE mouse monoclonal antibody, clone MARE-1, FITC

Applications Flow Cytometry: Use 50 µl of 5 µg/ml diluted antibody to label 2 x 10e7 cells in 100 µl.
Reactivities Rat
Conjugation FITC

Rat IgG1 (heavy chain) mouse monoclonal antibody, clone MARG1.2, Biotin

Applications ELISA: 500ng/ml.
Reactivities Rat
Conjugation Biotin

Rat IgG1 (heavy chain) mouse monoclonal antibody, clone MARG1.2, FITC

Applications Flow Cytometry: Use 50 µl of 5 µg/ml diluted antibody to label 106 cells in 100 µl.
Reactivities Rat
Conjugation FITC

Rat IgG1 (heavy chain) mouse monoclonal antibody, clone MARG1.2, HRP

Applications ELISA: 0.5 µg/ml.
Reactivities Rat
Conjugation HRP

Rat IgG1 (heavy chain) mouse monoclonal antibody, clone MARG1.2, Purified

Applications ELISA: 3-5 µg/ml.
This antibody may be used as a Coating antibody in ELISA in combination with clone MARK-1/MARL-15 as detection reagent and purified Rat IgG1 (Cat.-No SA013).
Reactivities Rat

Rat IgG2a (heavy chain) mouse monoclonal antibody, clone MARG2a-1, Biotin

Applications ELISA: 500ng/ml.
Reactivities Rat
Conjugation Biotin

Rat IgG2a (heavy chain) mouse monoclonal antibody, clone MARG2a-1, HRP

Applications ELISA: 0.5µg/ml.
Reactivities Rat
Conjugation HRP

Rat IgG2a (heavy chain) mouse monoclonal antibody, clone MARG2a-1, Purified

Applications ELISA: 1.25 µg/ml; This antibody may be used as a coating antibody in ELISA in combination with clone MARK-1/MARL-15 as detection reagent and purified rat IgA (SA021).
Reactivities Rat

Rat IgG2b (heavy chain) mouse monoclonal antibody, clone MARG2b-3, Biotin

Applications ELISA.
Reactivities Rat
Conjugation Biotin

Rat IgG2b (heavy chain) mouse monoclonal antibody, clone MARG2b-3, Purified

Applications ELISA: 5ug/ml; This antibody may be used as a coating antibody in ELISA in combination with clone MARK-1/MARL-15 as detection reagent and purified rat IgG2b (SA023).
Reactivities Rat

Rat IgM (heavy chain) mouse monoclonal antibody, clone MARM-4, Biotin

Applications ELISA: 125ng/ml as detecting antibody.
Reactivities Rat
Conjugation Biotin

Rat IgM (heavy chain) mouse monoclonal antibody, clone MARM-4, FITC

Applications Flow Cytometry: 10 µg/ml; use 50µl of the suggested working dilution to label 10e6 cells in 100µl.
Reactivities Rat
Conjugation FITC

Rat IgM (heavy chain) mouse monoclonal antibody, clone MARM-4, HRP

Applications ELISA: 0.5 µg/ml.
Reactivities Rat
Conjugation HRP

Rat IgM (heavy chain) mouse monoclonal antibody, clone MARM-4, Purified

Applications ELISA: 5 µg/ml as Coating antibody in combination with clone MARK-1/MARL-15 (AM05512HR-N) as Detection reagent and purified IgM (SA025).
Reactivities Rat

Feline IgG mouse monoclonal antibody, clone K62 2G4, Supernatant

Applications ELISA: 1/300 (detection).
Reactivities Feline

Mouse IgG1 (heavy chain) rat monoclonal antibody, clone LO-MG1-13, Purified

Applications ELISA: 5 µg/ml as coating antibody.
This antibody may be used as a Coating antibody in ELISA in combination with SM1017HRP as the detection reagent.
Reactivities Mouse

Mouse IgG2a (heavy chain) rat monoclonal antibody, clone LO-MG2a-9, FITC

Applications Flow cytometry: 5µg/ml;
Reactivities Mouse
Conjugation FITC

Mouse IgG2a (heavy chain) rat monoclonal antibody, clone LO-MG2a-9, Purified

Applications ELISA: 5µg/ml; This antibody may be used as a coating antibody in ELISA in combination with LO-MG2a-7 (SM1018HRP) as the detection reagent.
Reactivities Mouse

Mouse IgG2b (heavy chain) rat monoclonal antibody, clone LO-MG2b-1, Purified

Applications ELISA: 5 µg/ml; This antibody may be used as a coating antibody in ELISA in combination with LO-MK1 (SM1496P) as the detection reagent.
Reactivities Mouse

Mouse IgG3 (heavy chain) rat monoclonal antibody, clone LO-MG3-13, Purified

Applications ELISA (5 µg/ml): This antibody may be used as a Coating antibody in ELISA in combination with LO-MG3-7 (SM1022B) as the Detection reagents.
Reactivities Mouse

Rat IgG2b (heavy chain) mouse monoclonal antibody, clone MARG2b-8, Biotin

Applications ELISA: 500ng/ml.
Reactivities Rat
Conjugation Biotin

Rat IgG2b (heavy chain) mouse monoclonal antibody, clone MARG2b-8, Purified

Applications ELISA: 5 µg/ml (as a coating antibody).
Reactivities Rat

Equine IgGb mouse monoclonal antibody, clone CVS39, Purified

Applications ELISA.
Reactivities Equine

Human IgE mouse monoclonal antibody, clone IA2, HRP

Applications ELISA: 1/500 - 1/1000; This HRP conjugated antibody may be used as a detection reagent in a sandwich ELISA for IgE, utilising SM1814P as capture reagent.
Reactivities Human
Conjugation HRP

Human IgM (Fc-region) mouse monoclonal antibody, clone CH2, APC

Applications Flow Cytometry: 2 µg/ml is recommended starting point.
Reactivities Human
Conjugation APC

Human IgM (Fc-region) mouse monoclonal antibody, clone CH2, FITC

Applications Flow cytometry (1/500 starting dilution).
Reactivities Human
Conjugation FITC

Human IgM (Fc specific) mouse monoclonal antibody, clone CH2, PE

Applications Flow cytometry: 0.5 µg/ml.
Reactivities Human
Conjugation PE