Antibodies

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DYKDDDDK Epitope Tag chicken polyclonal antibody, Aff - Purified

Applications ELISA, IF, IHC, IP, WB
Immunogen Synthetic peptide containing the DYKDDDDK epitope conjugated to Keyhole Limpet Hemocyanin (KLH).
Production: The first injection included complete Freund’s adjuvant; subsequent injections included incomplete Freund’s adjuvant. Eggs were collected after the fourth injection, and antibodies were purified from the yolks using a proprietary method that yields a purity of >90%. Antibodies were then affinity-purified over a peptide column and dialyzed against Phosphate-Buffered isotonic Saline. Sodium azide was added, the protein concentration was adjusted and the antibodies were filter-sterilized (0.2 μm).

c-Myc (MYC) chicken polyclonal antibody, Aff - Purified

Applications ELISA, IF, IHC, IP, WB
Reactivities Human
Immunogen Synthetic peptide containing the Human c-myc epitope (i.e., EQKLISEEDL) conjugated to KLH.
The first injection included complete Freund's adjuvant; subsequent injections included incomplete Freund's adjuvant. Eggs were collected after the fourth injection, and antibodies were purified from the yolks using a proprietary method that yields a purity of >90%.

6xHistidine Epitope Tag (HHHHHH) chicken polyclonal antibody, Purified

Applications IP, WB
Immunogen 6X-Histidine peptide conjugated to Keyhole Limpet Hemocyanin protein and emulsified in Freund’s adjuvants.
After multiple injections, eggs were collected from the hens, IgY fractions were prepared from the yolks, and then affinity-purified antibodies were prepared using 6X-Histidine peptide conjugated to an agarose matrix.
To make up the final mixture, the IgY fraction was spiked with affinity purified antibody, mixed with Glycerol and Thimerosal, and filter-sterilized.

6xHistidine Epitope Tag (HHHHHH) chicken polyclonal antibody, Aff - Purified

Applications IP
Immunogen 6X-Histidine peptide conjugated to Keyhole Limpet Hemocyanin protein and emulsified in Freund’s adjuvants.
After multiple injections, eggs were collected from the hens, IgY fractions were prepared from the yolks, and then affinity-purified antibodies were prepared using 6X-Histidine peptide conjugated to an Agarose matrix.
The antibody's concentration was then adjusted, mixed with Glycerol and Thimerosal, and filter-sterilized.