Mouse MGC-Plus Clone Collection: Mouse Full-Length cDNA Clones

OriGene has expanded its TrueClone collection with the addition of a large collection of mouse full-length cDNA clones in a selectable mammalian expression vector. All Mouse Clones are derived from the NIH MGC Clone collection with significant improvement on the vector. The major differences are summarized below.

Search the entire OriGene TrueClone Mouse catalog by Gene Name, Gene Symbol, Accession Number or Keyword in the search box below

View Mouse cDNA Clones
SKU Description Availability
PCMV6KN Cloning vector PCMV6-Kan/Neo, 10 ug circular vector In Stock

TrueClone Mouse Vector map: download: Application Guide Cloning map and Vector sequence

KanNeo no sfiisite vector map

For genes cloned with Sfi I sites, the alternative vector (see below) is used.
Download: Vector Map and Cloning Map

Features of the TrueClone Mouse Vector

  1. The CMV promoter and a Kozak consensus sequence drive protein expression in mammalian cells.
  2. Ten (10) ug of purified plasmid is provided for immediate transfection and expression experiments.
  3. An antibiotic selection cassette (Kanr/ Neor) confers resistance to kanamycin in E. coli and neomycin analogs in mammalian cells.
  4. The T7 promoter upstream of the ORF allows protein expression in cell-free system.
  5. Each clone contains a fully verified insert sequence.

Example Verification of Protein Over-expression by Western blot

Mousenorthernblot

cDNA Clone Resources