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Cas9 Protein
Cas9 protein is an RNA-guided endonuclease that can be used for the site-specific cleavage of double stranded DNA. OriGene offers a complete set of Cas9 products from DNA clone to purified protein and specific antibody. All of them are created based on wild type Streptococcus pyogenes Cas9 protein.
Why Choose OriGene Cas9 Protein?
- Sterile protein with NLS signals
- High RNA-guided endonuclease activity
- High genome editing efficiency
- High protein concentration & purity
Featured products:
| SKU | Description | Manual | Size | Availability |
|---|---|---|---|---|
| TP790148 | CAS9 S. pyogenes Recombinant Protein | 500 pmol | In stock | |
| TP790151 | CAS9-D10A S. pyogenes Recombinant Protein | 500 pmol | In stock | |
| GE100053 | Cas9 in vitro digestion buffer, 10x | 0.2 ml | In stock |
Functional studies of wild type Cas9 protein (Cat. No. TP790148)
In vitro cleavage View Protocol
Figure 1: In-vitro digestion of PS100074 vector with purified Cas9 protein (Cat. No. TP790148) and specific sgRNA. Purified Cas9 protein can cut the substrate DNA only when it is incubated with specific sgRNA.
In vivo genome editing View Protocol
Figure 2: In-vivo genome editing of tRFP positive cells with purified Cas9 protein (Cat. No. TP790148) and specific sgRNA. Purified Cas9 protein can knockout the expression of tRFP protein in the tRFP postive cells when co-transefected with specific sgRNA.