MDA5 Pathway (RIG1, MAVS, IRF3, IFIT1, MDA5) Antibody Panel

MDA5( Melanoma differentiation-associated gene 5), encoded by gene IFIH1, is a member of the RLR family. It is a pattern-recognition receptor that recognizes viral RNA and induces type 1 interferon response. MDA5 plays a crucial role in responses to viral infections and in autoimmune and autoinflammatory diseases and cancer.

Most members of the RLR family have two RNA binding domains: the central helicase domain and the C terminal domain. Still, only MDA5 and RIG-I have an N terminal caspase activation and recruitment domain (CARDs) that leads to the Type I interferon response.

Recent studies have shown that MDA5 recognizes cellular RNA and viral RNAs. Mutation in the IFIH1 gene is associated with multiple autoimmune diseases, but the impact of the mutations in both viral sensing and autoimmune disease is a hot topic of investigation.

IFIT1

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-IFIT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100?C for 10min, TA500948)

MAV5

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MAVS mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120?C for 3min, TA808253) (1:150)

IRF3

Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-IRF3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100?C for 10min, TA500465, Dilution 1:50)

RIG-I

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-DDX58 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120?C for 3min, TA506141)

MDA5 Antibody Panel

• MAVS: TA808253S

  30 ul

  MAVS mouse monoclonal antibody, clone OTI9C4 (formerly 9C4)

• IRF3: TA500465S

  30 ul

  Anti-IRF3 mouse monoclonal antibody, clone OTI5D2 (formerly 5D2)

• IFIT1: TA500948S

  30 ul

  IFIT1 mouse monoclonal antibody, clone OTI3G8 (formerly 3G8)

• IFIH1: TA803667S

  30 ul

  IFIH1 (MDA5) mouse monoclonal antibody, clone OTI8C10 (formerly 8C10)

• DDX58: TA506141S

  30 ul

  DDX58 mouse monoclonal antibody, clone OTI6C1 (formerly 6C1)

MDA5 Pathway

MDA5 Pathway- When a virus enters the host, MDA5 is activated by binding to long molecules of double-stranded RNA generated during viral infection. MDA5 can get activated by synthetic RNA, viral RNA, and endogenous RNA. On activation, multiple CARD domains cluster leading to engagement of MAVS and induction of type1 IFNs.

RIG-I and MDA5

RIG-I (retinoic acid-inducible gene I) is a cytosolic pattern recognition receptor that is important for innate immunity. It is part of the RLR family similar to MDA5. Though they are similar in structure they respond to distinct species of RNA viruses. RIG-I responds to in-vitro transcribed dsRNA, vesicular stomatitis virus, influenza virus and Newcastle disease virus in mice. Whereas MDA5 recognizes poly(I:C) and picornavirus. Unlike MDA5, RIG-I recognizes ssRNA bearing 5? phosphatase.

Tools for MDA5 Research

Expression-validated cDNA clone

Proteins

Carrier-free antibodies