Plasmid Reconstitution Protocol

All OriGene plasmids are delivered as lyophilized plasmids and must be reconstituted before use. Read this short protocol or watch this video to learn more.

Download/Print this Protocol

Step 1, Spin: Spin the tube at 10,000g for 2 min.

Step 2, Add Sterile Water: Carefully open the tube and add 100 ul dH2O to produce a concentration of 100 ng/ul.

Note: Dissolving the plasmid DNA in a lower volume is not recommended as an increased EDTA concentration may affect some downstream applications.

Step 3, Incubate: Close the tube and let it sit for 10 minutes at room temperature, or 4?C overnight.

Step 4, Vortex: Vortex the tube briefly and then do a quick spin to concentrate the liquid at the bottom.

The DNA is ready for immediate use in:

  • Transfection into mammalian cells
  • Restriction enzyme digestion
  • Protein expression in cell-free systems
  • PCR amplification
  • Probe labeling
  • E. coli transformation for DNA amplification
  • Sequencing reactions