Rgs14 Mouse Monoclonal Antibody [Clone ID: N133/21]

CAT#: 75-170

Rgs14 mouse monoclonal antibody, clone N133/21

Product Images

Immunoblot against crude membranes from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] probed with N133/21 (left) or N52A/42 (right) TC supe

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  Immunoblot against lysates from wild-type (+/+) and RGS14 knockout (-/-) mouse brains probed with N133/21. Data courtesy of John Hepler (Emory).

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  Adult rat hippocampus immunohistochemistry

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  Adult rat brain membrane immunoblot

Specifications

Product Data

• Clone Name: N133/21
• Applications: IF, IHC, WB
• Recommended Dilution: Immunoblot (IB)
  Immunohistochemistry (IHC)
  Immunocytochemistry (ICC)
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Fusion protein amino acids 1-544 (full-length) of rat RGS14 (also known as Regulator of G-protein signaling 14, RAP1/RAP2-interacting protein and RPIP1, accession number O08773).
  Mouse: 95% identity (524/547 amino acids identical).
  Human: 86% identity (475/547 amino acids identical).
  <45% identity with RGS12 and other RGS proteins.
  
  Epitope mapped to within amino acids 444-490 (between 2nd Ras-binding & GoLoco domains, PDAKTREASSIPPCRSQGCLPRTQTKDSHLPPLSSSLSVEDASGSTG, 2014 Evans et al J Comp Neurol).
  Mouse: 85% identity (40/47 amino acids identical).
  Human: 51% identity (24/47 amino acids identical).
• Specificity: Does not cross-react with RGS12
• Formulation: State: Purified
• Conjugation: Unconjugated
• Gene Name: regulator of G-protein signaling 14
• Database Link:
  Entrez Gene 114705 Rat
  UniProt ID O08773
• Synonyms: RGS14
• Note: USERS will cite the UC Davis/NIH NeuroMab Facility in any publication(s) describing the research utilizing the MATERIALS. The suggested acknowledgment statement is as follows:
  "The monoclonal antibody _ was developed by and/or obtained from the UC Davis/NIH NeuroMab Facility, supported by NIH grant U24NS050606 and maintained by the Department of Neurobiology, Physiology and Behavior, College of Biological Sciences, University of California, Davis, CA 95616."
  Also, please include the complete clone number (e.g., N52A/42) and the Antibody Registry identification number (e.g., RRID:AB_2120479) to avoid ambiguity.
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