E Cadherin (CDH1) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 22F8]

CAT#: AM00010BT-N

E Cadherin (CDH1) (incl. pos. control) mouse monoclonal antibody, clone 22F8, Biotin

Specifications

Product Data

• Clone Name: 22F8
• Applications: WB
• Recommended Dilution: Immunoblotting: 0.5 µg/ml for HRPO/ECL detection.
  Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
  Included Potitive Control: Cell lysate from untreated A431 (See Protocols for more details).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Peptide conjugated to hemocyanin.
  Epitope: Cytoplasmic domain.
• Specificity: This monoclonal antibody specifically recognizes E-Cadherin at 124 kDa.
• Formulation: PBS containing 0.09% Sodium Azide, PEG and Sucrose.
  Label: Biotin
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Subsequent Ultrafiltration and Size Exclusion Chromatography.
• Conjugation: Biotin
• Storage: Aliquote and freeze in liquid nitrogen.
  Antibody can be stored frozen at -80°C up to 1 year.
  Thaw aliquots at 37°C.
  Thawed aliquots may be stored at 4°C up to 3 months.
• Gene Name: Homo sapiens cadherin 1 (CDH1), transcript variant 1
• Database Link:
  Entrez Gene 999 Human
• Background: Cadherins are a family of transmembrane glycoproteins that play a key role in calcium-dependent cell-cell adhesion. Several members of the cadherin family have been identified so far, including E-(epithelial), P-(placental), N-(neuronal), and M- muscle) cadherin. E-cadherin is expressed
  in most epithelial tissues.
• Synonyms: Epithelial cadherin, E-cadherin, Uvomorulin, CAM 120/80, CDH1, CDHE, UVO
• Note: Protocol: Positive Control: Cell lysate from untreated A431 cells, epidermoid carcinoma (Human)
  
  Format: Lyophilized cell lysate from serum starved A431 cells.
  
  Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.
  
  Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells.
  Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins.
  Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended for applications with native proteins such as in immunoprecipitation.
  
  Storage: Aliquote reconstituted product and store frozen. Avoid repeated fereezing and thawing.

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Adherens junction, Bladder cancer, Cell adhesion molecules (CAMs), Endometrial cancer, Melanoma, Pathogenic Escherichia coli infection, Pathways in cancer, Thyroid cancer