GST-Tag Mouse Monoclonal Antibody [Clone ID: 4H3]
Specifications
Product Data | |
Clone Name | 4H3 |
Applications | ELISA, IP, WB |
Recommended Dilution | Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. ELISA: 0.05 µg/ml. Immunoprecipitation: 1-10 µg/ml. |
Reactivities | Schistosoma japonicum |
Host | Mouse |
Isotype | IgG2a |
Clonality | Monoclonal |
Immunogen | Recombinant GST (pGEX), Schistosoma japonicum |
Specificity | This antibody specifically interacts with GST of Schistosoma japonicum that is encoded by the pGEX expression vectors. The antibody does not interact with mammalian GST proteins. |
Formulation | 1 ml 2xPBS / 0.09% Sodium Azide / PEG and Sucrose State: Purified State: Lyophilized purified IgG fraction |
Reconstitution Method | Restore with 1 ml H2O (15 min, RT). |
Purification | Size Exclusion Chromatography |
Background | The glutathione-S-transferase (GST) of Schistosoma japonicum is widely used as fusion partner in protein expression systems. The GST can be used for affinity purification of fusion proteins on immobilized glutathion as well as tag sequence if antibodies specific for the expressed protein are not available. |
Synonyms | Glutathione S-transferase Tag, GST26-Tag |
Reference Data |
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