Phosphoserine (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 4H4]

CAT#: AM00115PU-N

Phosphoserine (incl. pos. control) mouse monoclonal antibody, clone 4H4, Purified

Product Images

Phosphoserine Detection: Phosphoprotein Positive Control was probed with Lane 1: mab 1C8 (IgM), 1 g/ml Lane 2: mab 4A3 (IgM), 1 g/ml Lane 3: mab 4A9 (IgM), 1 g/ml Lane 4: mab 4H4 (IgM), 1 g/ml Lane 5: mab 7F12 (IgG), 1 g/ml Lane 6: mab 16B4 (IgM), 1 g/ml

Specifications

Product Data

• Clone Name: 4H4
• Applications: ELISA, IP, WB
• Recommended Dilution: Western Blot: 1 µg/ml for HRPO/ECL detection.
  Recommended blocking buffer: BSA/Tween 20 based blocking buffer. DO NOT USE MILK OR CASEIN FOR BLOCKING!
  ELISA: 0.05 µg/ml.
  Immunoprecipitation: 1-10 µg per 10e6 pervanadate-treated A431 cells.
  Included Positive Control: Phosphoserine/phosphothreonine positive control.
• Reactivities: Canine, Human, Mouse, Rat
• Host: Mouse
• Isotype: IgM
• Clonality: Monoclonal
• Immunogen: Synthetic phosphopeptide conjugated to KLH
• Specificity: This antibody recognizes a broad range of Serine-phosphorylated proteins in crude cell extracts, preferring positively charged amino acids directly neighboured to Phosphoserine.
  Please note that Phosphoserine detection by monoclonal antibodies is always dependent on the surrounding amino acid sequence!
• Formulation: 1ml 2xPBS containing 0.09% Sodium Azide, PEG and Sucrose
  State: Purified
  State: Lyophilized purified IgG fractio from Serum-free cell culture supernatant
• Reconstitution Method: Restore with 1.0 ml H2O (15 min, RT).
• Purification: Size Exclusion Chromatography
• Background: Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein-interactions. Modification of proteins on serine residues is mediated by serine/threonine kinases.