Tlr2 Rat Monoclonal Antibody [Clone ID: 6C2]

CAT#: AM01267PU-S

Tlr2 rat monoclonal antibody, clone 6C2, Purified

Specifications

Product Data

• Clone Name: 6C2
• Applications: FC, IF, IP
• Recommended Dilution: Flow cytometry.
  Immunoflourescence of fixed RAW cells: The typical starting working dilution is 1:50.
  Immunoprecipitation.
  Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added.
  Not useful for Western blot.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Specificity: Monoclonal antibody 6C2 reacts with mouse Toll-like receptor 2 (TLR2, CD282). It gives only a small inhibition of TLR2 (CD282) ligands in RAW cells.
• Formulation: PBS
  State: Purified
  State: Liquid 0.2 µm filtered Ig fraction
  Stabilizer: 0.1% bovine serum albumin
  Preservative: 0.02% sodium azide
• Concentration: 0.1 mg/ml
• Purification: Protein G
• Gene Name: toll-like receptor 2
• Database Link:
  Entrez Gene 24088 Mouse
• Background: Toll-like receptors (TLR) are highly conserved throughout evolution and have been implicated in the innate defense to many pathogens. In Drosophila toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defenses. In mammals, TLR identified as type I transmembrane signaling receptors with pattern recognition capabilities, have been implicated in the innate host defense to pathogens. TLR2 has been identified as a receptor that is central to the innate immune response to lipoproteins of gram-negative bacteria, several whole gram-positive bacteria, as well as a receptor for peptidoglycan and lipoteichoic acid and other bacterial cell membrane products. A functional interaction between TLR2 and TLR6 in the cellular response to various bacterial products has been discovered. The currently accepted paradigm regards TLR2 as an essential receptor for many eubacterial cell wall components, including lipoproteins and peptidoglycan. Bacterial species as diverse as mycobacteria, spirochetes, mycoplasma, Staphylococcus aureus, and Streptococcus pneumoniae have all been shown to mediate cellular activation via TLR2.
• Synonyms: Toll-like receptor 2