HLAG (HLA-G) (C-term) Mouse Monoclonal Antibody [Clone ID: 5A6G7]

CAT#: AM03081PU-N

HLAG (HLA-G) (C-term) mouse monoclonal antibody, clone 5A6G7, Purified

Product Images

Western Blotting analysis of whole cell lysate of HLA-G stable transfectants (various splice variants) using anti-human HLA-G (5A6G7). Lane 1: M8 cell line transfected with empty vector; Lane 2: M8 cell line transfected with HLA-G1; Lane 3: M8 cell line transfected with HLA-G5; Lane 4: M8 cell line transfected with HLA-G6

Specifications

Product Data

• Clone Name: 5A6G7
• Applications: ELISA, FC, IF, IHC, WB
• Recommended Dilution: Western blot (Reducing Conditions): 12% AA SDS-PAGE.
  Positive Control: JEG-3 cell lysate.
  Flow Cytometry: 2 µg/ml.
  Immunohistochemistry on Paraffin and Frozen Sections: 10-20 μg/ml.
  Positive tissue: placenta tissue.
  Immunocytochemistry.
  ELISA: The antibody 5A6G7 has been tested as the capture antibody in a sandwich ELISA for analysis of soluble HLA-G in combination with antibody AM03005BT-N.
  Positive Control:
  HeLa/HLA-G5 transfectants cell lysate.
  HeLa/HLA-G5 cell supernatant.
  Negative Control: HeLa cell lysate.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: C-terminal amino acid sequence (22-mer) of soluble HLA-G5 and HLA-G6 proteins coupled to ovalbumin
• Specificity: The antibody 5A6G7 allows to discriminate between soluble HLA-G protein yielded by shedding from membrane-bound HLA-G forms that do not contain the intron 4-encoded epitope and soluble HLA-G5/HLA-G6 produced from intron 4-retaining alternatively spliced mRNAs. HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells.
• Formulation: PBS, pH 7.4 with 15 mM Sodium Azide as preservative
  State: Purified
  State: Liquid purified IgG fraction (> 95% pure by SDS-PAGE)
• Concentration: 1.0 mg/ml
• Purification: Protein A chromatography
• Database Link:
  Entrez Gene 3135 Human
• Background: HLA G belongs to the HLA class I heavy chain paralogues. It is a heterodimer consisting of a heavy chain (with a1, a2, a3 domains) and a light chain (beta 2 microglobulin). Alternate splicing provides both soluble and membrane bound forms of HLA G, with two soluble isoforms and four membrane bound isoforms. HLA G may be part of the fetal protection system and is expressed on fetal derived placental cells.
• Synonyms: HLA-6.0, HLAG, MHC class I antigen G