CD44 (21-145) Mouse Monoclonal Antibody [Clone ID: 5C10]

CAT#: AM03178PU-N

CD44 (21-145) mouse monoclonal antibody, clone 5C10, Purified

Product Images

The Cell lysates of U937 (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CD44 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Western blot analysis: The Cell lysates of U937 (30 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CD44 antibody (1/1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Immunohistochemistry: Cd44 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Breast at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Specifications

Product Data

• Clone Name: 5C10
• Applications: ELISA, FC, IF, IHC, WB
• Recommended Dilution: ELISA.
  Western blot: 1/1,000-1/2,000.
  Flow Cytometry.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Immunofluorescence/Immunocytometry.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Recombinant Human CD44 (aa 21-145) purified from E. coli
• Specificity: This antibody detects Human CD44 / HUTCH-I.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Protein-G affinity Chromatography
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 960 Human
• Background: CD44 is a broadly distributed transmembrane glycoprotein that plays a critical role in a variety of cellular behaviors, including adhesion, migration, invasion, and survival. CD44 mediates cell-cell and cell-matrix interactions in a large part through its affinity for hyaluronan (HA), a glycosaminoglycan constituent of extracellular matrices, but also potentially through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Variants of CD44 are expressed in tissues during development, including embryonic epithelia. Known functions of CD44 are cellular adhesion (aggregation and migration), hyaluronate degradation, lymphocyte activation, lymph node homing, myelopoiesis and lymphopoiesis, angiogenesis, and release of cytokines. The functions of CD44 are principally dependant on cellular adhesion in one setting or another.
• Synonyms: LHR, MDU2, MDU3, MIC4, CDw44, Epican, ECMR-III, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, PGP-1