CD45 (PTPRC) (CD45RA) Mouse Monoclonal Antibody [Clone ID: BHKG-2]

CAT#: AM05193PU-N

CD45 (PTPRC) (CD45RA) mouse monoclonal antibody, clone BHKG-2, Purified

Specifications

Product Data

• Clone Name: BHKG-2
• Applications: FC
• Recommended Dilution: Flow Cytometry.
  Monitoring of Leucocyte cells subsets in peripheral blood.
  Characterization of subtypes of leukemias and lymphomas.
  Studies of AIDS virus infection.
  Studies of multiple sclerosis and other autoimmune disease.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells of BALB/c mice immunized with peripheral blood monocytes from rheumatoid arthritis patient
• Specificity: CD45RA recognizes Mr. 220-kda of the Leucocyte Common Antigen (LCA).
• Formulation: PBS containing 0.08% Sodium Azide as preservative.
  State: Purified
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Database Link:
  Entrez Gene 5788 Human
• Background: CD45RA antigen is a member of the CD45 antigen family that also includes CD45, CD45RO, and CD45RB antigens. CD45RA is expressed on approximately 75% of CD8 lymphocytes and 50% of CD4 lymphocytes, all B cells and NK Cells.
  CD45RA antigen is expressed on naive T lymphocytes, antigen density decreases upon in vitro activation. A decrease antigen density has been demonstrated for CD4+CD45RA+subset with active multiple sclerosis.
• Synonyms: PTPRC, Leukocyte common antigen, L-CA, T200
• Note: Protocol: PBMC:
  Add 10 ul of MAB/10e6 PBMC in 100 ul PBS.
  Mix gently and incubate for 15 minutes at 2-8°C.
  Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.
  
  WHOLE BLOOD:
  Add 10 ul of MAB/100 ul of whole blood.
  Mix gently and incubate for 15 minutes at room temperature (20°C).
  Lyse the whole blood.
  Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.
  See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.