RAF1 Mouse Monoclonal Antibody [Clone ID: 4G4]

CAT#: AM06729PU-N

RAF1 mouse monoclonal antibody, clone 4G4, Purified


USD 425.00

2 Weeks*

Size
    • 100 ug

Specifications

Product Data
Clone Name 4G4
Applications ELISA, FC, IHC, WB
Recommended Dilution ELISA: 1/10000.
Western Blot: 1/500 - 1/2000.
Flow Cytometry: 1/200 - 1/400.
Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
Reactivities Human
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Purified recombinant fragment of Human RAF1 expressed in E. Coli.
Specificity This antibody recognizes RAF1
Formulation State: Purified
State: Liquid purified antibody in PBS containing 0.05% Sodium Azide and 0.5% protein stabilizer
Concentration lot specific
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Background This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
Synonyms C-RAF, Raf-1, cRaf
Note Tissue Array Results: Hepatic Carcinoma (+, Cytoplasm, B5/6), Skeletal muscle (+, Cytoplasm, H7/8), Liver (+, Cytoplasm, C5/6).
Reference Data

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.