Cd80 Rat Monoclonal Antibody [Clone ID: 1G10]

CAT#: AM08052PU-N

Cd80 rat monoclonal antibody, clone 1G10, Purified

Specifications

Product Data

• Clone Name: 1G10
• Applications: FC, FN, IP
• Recommended Dilution: Flow Cytometry. (Ref.2,7)
  Immunoprecipitation. (Ref.7)
  Functional Assays: In vivo and In vitro blocking of ligand binding. (Ref.7)
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Specificity: This antibody recognises CD80/B7-1 (Mr. 45-47 kDa).
  1G10 blocks binding of CTLA-4 to CD80, but it does not block stimulation of T cells by natural antigenpresenting cells. (Ref.2,7).
• Formulation: 100 mM Borate Buffered Saline, pH 8.2.
  No preservatives or amine-containing buffer salts added.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: 0.5 mg/ml
• Database Link:
  Entrez Gene 12519 Mouse
• Background: CD80 (B7-1) is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed by dendritic cells, monocytes, and peritoneal macrophages. Expression of CD80 on B cells is upregulated after activation by various means including stimulation by LPS, IL-4, and surface Ig receptor cross linking with peak expression occurring between 48 and 72 hours postactivation. (Ref.1-4)
  The interaction of CD80/B7-1 with its ligands, CD28 and CD152/CTLA-4, provides a potent costimulatory signal for T cells activated through the CD3/T cell receptor complex. (Ref.5,6)
• Synonyms: CD28LG, CD28LG1, LAB7, BB1, B7.1, B7-1