Ly6c1 Rat Monoclonal Antibody [Clone ID: HK1.4]

CAT#: AM08056PU-N

Ly6c1 rat monoclonal antibody, clone HK1.4, Purified

Product Images

Immunofluorescent Staining: Bone marrow cells from C57BL/6 mice were labeled with purified Rat anti-Mouse Ly-6C and then stained with Goat anti-Rat IgG-FITC. Large cells were gated and analyzed by flow cytometry. Amount Used: 1 ug/10e6 cells.

Specifications

Product Data

• Clone Name: HK1.4
• Applications: FC, FN, IHC
• Recommended Dilution: Flow Cytometry: < / = 1 µg/10e6 cells. (Ref.1,2)
  Immunohistochemistry on Frozen Sections. (Ref.5)
  In vitro Activation of T Lymphocytes. (Ref.1,2)
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2c
• Clonality: Monoclonal
• Specificity: This antibody is specific to Murine Ly-6C (Mr. 18 kDa)
• Formulation: 100 mM Borate Buffered Saline, pH 8.2.
  No preservatives or amine-containing buffer salts added.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 17067 Mouse
  UniProt ID P0CW02
• Background: Ly-6C is a member of the Ly-6 multigene family of type V glycophosphatidylinositol-anchored cell surface proteins. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T-cell subsets. Mice with the Ly-6.2 allotype (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have subsets of CD4+Ly-6C+ and CD8+Ly-6C+ cells, while Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have only CD8+Ly-6C+ lymphocytes. Ly-6C may play a role in the development and maturation of lymphocytes. (Ref.1-4)
• Synonyms: Lymphocyte antigen 6C2, Ly-6C2, Ly6C2, Mouse Macrophage Marker