Itgb7 Rat Monoclonal Antibody [Clone ID: DATK32]

CAT#: AM08074PU-N

Itgb7 rat monoclonal antibody, clone DATK32, Purified

Product Images

Immunofluorescent Staining: Either the TK-1 cell line (LPAM-1 expressing) or the 18-81 MuLV-transformed pre-B cell line (LPAM-1 negative) was labeled with Rat anti-Mouse LPAM-1 and then stained with Goat anti-Rat IgG-FITC. The primary cell populations on each scatter plot were then gated and analyzed by flow cytometry. Amount Used: 1 Ã?ug/10e6 cells.

Specifications

Product Data

• Clone Name: DATK32
• Applications: FC, FN, IHC
• Recommended Dilution: Flow Cytometry: ≤ 1 µg/10⁶ cells. (Ref.1-4)
  Immunohistochemistry on Frozen Sections. (Ref.3)
  Induction of homotypic cellular aggregation. (Ref.1)
  Blockade of binding to cell adhesion molecules. (Ref.1)
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: AKR/Cum mouse spontaneous T lymphoma cell line TK1. (Ref.1)
• Specificity: This antibody is specific to mouse LPAM1 (Integrin alpha 4 beta 7).
  It recognises a conformational epitope on the heterodimer.
• Formulation: 100 mM Borate Buffered Saline, pH 8.2.
  No preservatives or amine-containing buffer salts added.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: 0.5 mg/ml
• Gene Name: Mus musculus integrin beta 7 (Itgb7)
• Database Link:
  Entrez Gene 16421 Mouse
• Background: The lymphocyte Peyer’s patch adhesion molecule (LPAM-1), also known as Integrin alpha 4 beta 7 is a member of the integrin family of cell surface receptors. It is expressed primarily on mucosal lymphocytes, but is also present on NK cells and eosinophils. The alpha 4 beta 7 heterodimer mediates the binding of lymphocytes to its ligand, mucosal vascular addressin (MAdCAM1) on the high endothelial venules, thereby directing the homing of lymphocytes into Peyer's patches and intestinal lamina propria. (Ref.1-4)
• Synonyms: Integrin beta-P, M290 IEL antigen, ITGB7, LPAM-1, LPAM1