CD14 Rabbit Monoclonal Antibody [Clone ID: EPR3653]

CAT#: AM10056SU-N

CD14 rabbit monoclonal antibody, clone EPR3653, Supernatant

Product Images

Formalin-Fixed, Paraffin-Embedded Human lymph node stained with CD14 antibody Cat.-No. AM10056SU-N using peroxidase conjugate and DAB chromogen. Note cytoplasmic and membrane staining of Langerhan cells (in follicle) and histiocytes in paracortical region.

Specifications

Product Data

• Clone Name: EPR3653
• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: A band at approximately 53kDa is detected.
  Immunocytochemistry.
  Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/100
  Pretreatment of deperaffinized tissue with heat-induced epitope retrieval is recommended.
  Use Polymer anti Mouse/Rabbit IgG as a detection system.
  Positive Control: Tonsil or lymph node.
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide corresponding to residues in Human CD14
• Specificity: This antibody labels monocyte-macrophages and Langerhans cells in Langerhans cell histiocytosis.
  Cellular Localization: Membrane and cytoplasmic.
• Formulation: State: Supernatant
  State: Tissue Culture Supernatant with 0.2% BSA and 15mM Sodium Azide.
• Storage: Store the antibody undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens B-cell CLL/lymphoma 6 (BCL6), transcript variant 2
• Database Link:
  Entrez Gene 604 HumanEntrez Gene 929 Human
• Background: CD14 is a surface protein preferentially expressed on monocytes/macrophages. It binds lipopolysaccharide binding protein and recently has been shown to bind apoptotic cells. Anti-CD14 antibody labels Kupffer cells in liver sinusoids. In lymphoid tissues, dendritic cells are distinctly stained. Most other normal tissues are negative. Tumor cells are positive in monocytic leukemia and true histiocytic lymphomas for CD14. Sinusoidal histiocytes express CD14 and CD 169, whereas most of the other monocyte-derived cells in reactive lymph node lack these markers. Anti-CD14 labels numerous diffuse large B-cell lymphomas and splenic marginal zone lymphoma but not in other B-cell lymphomas.
• Synonyms: CD14

Reference Data

• Protein Families: Druggable Genome, Transcription Factors