MSH2 Mouse Monoclonal Antibody [Clone ID: G219-1129]

CAT#: AM10116SU-N

MSH2 mouse monoclonal antibody, clone G219-1129, Supernatant

Product Images

Formalin-Fixed, Paraffin-Embedded Human colon adenocarcinoma stained with MSH2 antibody Cat.-No. AM10116SU-N using peroxidase conjugate and DAB chromogen. Note the nuclear staining of tumor cells.

Specifications

Product Data

• Clone Name: G219-1129
• Applications: IHC
• Recommended Dilution: Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/501/100
  Pretreatment of deperaffinized tissue with heat-induced epitope retrieval is recommended.
  Use Polymer anti Mouse/Rabbit IgG as a detection system.
  Positive Control: Colon carcinoma.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: BALB/C mice were injected with recombinant Human MSH2 protein.
• Specificity: Recognizes MSH2
  Cellular Localization: Nuclear.
• Formulation: State: Supernatant
  State: Tissue Culture Supernatant with 0.2% BSA and 15mM Sodium Azide.
• Storage: Store the antibody undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens microphthalmia-associated transcription factor (MITF), transcript variant 4
• Database Link:
  Entrez Gene 4286 HumanEntrez Gene 4436 Human
• Background: Germline mutations in human mismatch repair genes (hMSH2, hMSH6, hMLH1, hPMS2) account for majority of the hereditary non-polyposis colorectal carcinoma (HPNCC). CpG dinucleotides in the hMSH2 and hMLH1 genes are hotspots for HNPCC mutations. These mutations cause a mismatch repair deficiency resulting in a mutator phenotype where the replication errors are not repaired. Microsatellites / simple repeatative sequences are prone to this type of replication errors and instability of these microsatellites correlates with the occurance of HPNCC. hMSH2 binds to another MutS homolog protein GTBP to form a heterodimeric complex called hMutSbeta, which binds to insertion/deletion loops in DNA.
• Synonyms: DNA mismatch repair protein Msh2, MutS protein homolog 2

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Melanogenesis, Melanoma, Pathways in cancer