CD162 (SELPLG) Mouse Monoclonal Antibody [Clone ID: TC2]

CAT#: AM12002PU-N

CD162 (SELPLG) mouse monoclonal antibody, clone TC2, Aff - Purified

Product Images

Surface staining of human peripheral blood cells with anti-human CD162 (clone TC2).

Specifications

Product Data

• Clone Name: TC2
• Applications: FC, IHC
• Recommended Dilution: Flow Cytometry: 1.5-2.5 µg/ml.
  Positive Control: Human peripheral blood.
  This clone has been described to work in Immunohistochemistry on Paraffin Sections.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human thymocytes.
• Specificity: The antibody TC2 reacts with CD162, a 220 kDa type I integral membrane protein expressed as disulfide-linked homodimer (sialomucin family). CD162 is present on the most peripheral blood T lymphocytes, monocytes, granulocytes; it is also expressed on a subpopulation of B lymphocytes and CD34+ bone marrow cells.
• Formulation: PBS, pH~7.4
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pur by SDS-PAGE)
  Preservative: 15 mM Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens selectin P ligand (SELPLG), transcript variant 2
• Database Link:
  Entrez Gene 6404 Human
• Background: CD162 (P-selectin glycoprotein ligand-1, PSGL-1) is a sialomucin constitutively expressed as a disulfide-linked homodimer of two 120 kDa subunits on the surface of circulating leukocytes. CD162 serves as a ligand for P- E- and L-selectin, with the highest affinity for P-selectin. It is thus involved in leukocyte rolling at the endothelial surfaces, prerequisite for firm leukocyte adhesion and subsequent transendothelial migration. CD162 also mediates leukocyte-platelet adhesion and interleukocyte contacts. Whereas serving as an adhession molecule on mature leukocytes, CD162 is a potent negative regulator of human hematopoietic progenitors.
• Synonyms: PSGL-1, Selectin P ligand, SELPLG

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)